Interaction of glyceryl trinitrate and sodium nitroprusside with bovine pulmonary vein homogenate and 10,000 x g supernatant: biotransformation and nitric oxide formation.
Interaction of glyceryl trinitrate and sodium nitroprusside with bovine pulmonary vein homogenate and 10,000 x g supernatant: biotransformation and nitric oxide formation.
复制标题
三硝酸甘油和硝普钠与牛肺静脉匀浆和 10,000 x g 上清液的相互作用：生物转化和一氧化氮形成。
DOI：
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发表时间：
1991
影响因子：
2.1
通讯作者：
J. Brien
中科院分区：
文献类型：
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作者：
G. S. Marks;B. McLaughlin;L. B. Brown;D. Beaton;B. P. Booth;K. Nakatsu;J. Brien
目前提出的硝基血管扩张剂药物的作用机制涉及生物转化为一氧化氮，这被假定为活性血管扩张物质。我们的目的是确定是否形成一氧化氮从两个原型硝基血管扩张药物，硝酸甘油（GTN）和硝普钠（SNP），孵育后与牛肺静脉（BPV）的准备。将GTN或SNP在氩气气氛中与磷酸盐缓冲液、BPV匀浆或10，000 x g匀浆的上清液部分一起孵育。通过荧光-顶空气体法测定的一氧化氮形成在SNP与BPV匀浆和10，000 x g上清液孵育后是可测量的。有没有检测到形成一氧化氮从孵育的GTN与两个BPV制剂，虽然GTN生物转化为甘油二硝酸酯，通过气液色谱法测定。与缓冲液相比，在用两种BPV制剂孵育真实一氧化氮的过程中，一氧化氮的回收率降低。总之，一氧化氮的形成被测量为SNP而不是GTN与BPV制剂的相互作用。然而，这些数据并不排除GTN可能形成一氧化氮，因为一氧化氮被BPV制剂螯合或转化。
The current proposed mechanism of action of nitrovasodilator drugs involves biotransformation to nitric oxide, which is postulated to be the active vasodilator substance. Our objective was to determine whether nitric oxide was formed from two prototype nitrovasodilator drugs, glyceryl trinitrate (GTN) and sodium nitroprusside (SNP), after incubation with bovine pulmonary vein (BPV) preparations. GTN or SNP was incubated in an argon atmosphere with phosphate buffer, BPV homogenate, or the 10,000 x g supernatant fraction of the homogenate. Nitric oxide formation, as determined by a chemiluminescence-headspace gas method, was measurable following the incubation of SNP with BPV homogenate and 10,000 x g supernatant. There was no detectable formation of nitric oxide from the incubation of GTN with the two BPV preparations, although GTN was biotransformed to glyceryl dinitrate, as determined by gas-liquid chromatography. There was decreased recovery of nitric oxide during the incubation of authentic nitric oxide with the two BPV preparations as compared with buffer. In conclusion, formation of nitric oxide was measured for the interaction of SNP, but not GTN, with BPV preparations. However, the data do not exclude the possible formation of nitric oxide from GTN, as nitric oxide was shown to be sequestered or transformed by the BPV preparations.