In vitro cell invasion of Mycoplasma gallisepticum
In vitro cell invasion of Mycoplasma gallisepticum
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DOI：
10.1128/iai.68.7.4238-4244.2000
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发表时间：
2000-07-01
影响因子：
3.1
通讯作者：
Citti, C
中科院分区：
文献类型：
--
作者：
Winner, F;Rosengarten, R;Citti, C
广泛传播的禽病原体鸡毒支原体侵入培养的人上皮细胞的能力应用庆大霉素侵袭试验和免疫荧光双重显微镜技术对HeLa-229细胞和鸡胚成纤维细胞（CEF）进行了细胞内支原体的准确定位研究。内化的支原体能够离开细胞，但也能在细胞内存活48小时。两种细胞系的侵袭频率不同，但也相当依赖于支原体输入群体。在原型菌株R中，人工培养基中的低传代群体R-低能够进行主动细胞侵袭，而高传代群体R-高显示出对HeLa-229和CEF的粘附，但几乎没有摄取。通过使R-low和R-high通过HeLa-229细胞多次传代，侵袭频率显著增加。综上所述，这些研究结果表明，M。鸡败血症病毒具有进入非吞噬宿主细胞的能力，这可能为该病原体提供抵抗宿主防御和选择性抗生素治疗的机会，建立慢性感染，并穿过呼吸道粘膜屏障引起全身感染。
The ability of the widespread avian pathogen Mycoplasma gallisepticum to invade cultured human epithelial cells (HeLa-229) and chicken embryo fibroblasts (CEF) was investigated by using the gentamicin invasion assay and a double immunofluorescence microscopic technique for accurate localization of cell-associated mycoplasmas, The presence of intracellular mycoplasmas in both cell lines was clearly demonstrated, with organisms entering the eukaryotic cells within 20 min. Internalized mycoplasmas have the ability to leave the cell, but also to survive within the intracellular space over a 48-h period. Frequencies of invasion were shown to differ between the two cell lines, but were also considerably dependent on the mycoplasma input population. Of the prototype strain R, a low-passage population in artificial medium, R-low, was capable of active cell invasion, while a high-passage population, R-high, showed adherence to but nearly no uptake into HeLa-229 and CEF. By passaging R-low and R-high multiple times through HeLa-229 cells, the invasion frequency was significantly increased. Taken together, these findings demonstrate that M. gallisepticum has the capability of entering nonphagocytic host cells that may provide this pathogen with the opportunity for resisting host defenses and selective antibiotic therapy, establishing chronic infections, and passing through the respiratory mucosal barrier to cause systemic infections.