Determination of airborne particle-associated benz[a]anthracene-7,12-quinone using high-performance liquid chromatography with in-line reduction and fluorescence detection
Determination of airborne particle-associated benz[a]anthracene-7,12-quinone using high-performance liquid chromatography with in-line reduction and fluorescence detection
复制标题
采用在线还原和荧光检测的高效液相色谱法测定空气中颗粒相关的苯并[a]蒽-7,12-醌
DOI：
10.1016/j.chroma.2009.08.009
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
K. Hayakawa
中科院分区：
文献类型：
--
作者：
T. Kameda;T. Goto;A. Toriba;N. Tang;K. Hayakawa
建立了一种基于二维高效液相色谱-荧光检测器（HPLC-Fluorescence detection，FLC）测定大气颗粒物中苯并[a]蒽-7，12-醌（BaAQ）的方法。该系统包括用于样品净化的ODS柱、用于捕获含有BaAQ的馏分的ODS柱、用于样品分离的ODS柱和用于将BaAQ还原成相应荧光化合物的Pt-Rh催化柱。应用于加标已知量BaAQ的空气颗粒样品提取物的测定准确度为94- 108%。方法的检出限为97 fmol/次（信噪比=3），线性范围为1 ~ 100 pmol，相关系数R2=0.9995。在日本大坂每3小时收集的空气中的颗粒物中的BaAQ成功地测量使用建立的分析方法与简单的样品制备步骤：在有机溶剂中超声提取和减压和/或氮气流下浓缩。
A simple and sensitive method for measuring airborne particle-associated benz[a]anthracene-7,12-quinone (BaAQ) based on two-dimensional high-performance liquid chromatography (HPLC) with fluorescence detection was established. The system involves an ODS column for sample clean up, a 6-port switching valve, an ODS column for trapping the fraction containing BaAQ, an ODS column for sample separation, and a Pt-Rh catalytic column for reduction of BaAQ to a corresponding fluorescent compound. The accuracy of the assay, as applied to airborne particulate sample extracts spiked with known amounts of BaAQ was 94–108%. The detection limit was 97fmol per injection (signal-to-noise ratio=3), and the calibration range was from 1 to 100pmol with excellent proportionality (R2=0.9995). BaAQ in airborne particles collected in Osaka, Japan every 3h was successfully measured using the established analytical method with simple sample preparation steps: ultrasonic extraction in organic solvent and concentration under reduced pressure and/or nitrogen stream.