Heteromeric interactions of ripening-related ethylene receptors in tomato fruit
Heteromeric interactions of ripening-related ethylene receptors in tomato fruit
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DOI：
10.1093/jxb/erac314
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发表时间：
2022-07-21
影响因子：
6.9
通讯作者：
Klee, Harry J.
中科院分区：
文献类型：
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作者：
Kamiyoshihara, Yusuke;Achiha, Yuki;Klee, Harry J.
在番茄果实中，乙烯受体在跨亚家族的异构体复合体中发挥作用。这一发现为番茄果实成熟过程中乙烯信号的调控提供了洞察力。当气态植物激素乙烯被细胞感知时，跃变型果实的成熟就开始了。乙烯与膜相关的乙烯受体(ETRs)结合，通过多种成分触发一系列生化反应，导致大量成熟相关基因的诱导。在番茄中，ETR家族有7个成员，每个成员都参与果实成熟的调节。然而，每个受体对乙烯信号的相对贡献仍不清楚。在这里，我们展示了番茄果实中两个ETR亚家族异构体受体复合体的形成。亚家族II的免疫共沉淀导致亚家族I(SlETR1、SlETR2和SlETR3)的共纯化，但亚家族II成员(SlETR5、SlETR6和SlETR7)的共纯化。这种有偏见的相互作用在酵母双杂交试验中得到了验证，并在转基因拟南芥中得到了验证，在这些植物中，异源SLETR4与亚家族I ETR相互作用。我们的分析还显示，受体复合体与Raf样蛋白激酶SLCTR1和SLCTR3结合，这两个蛋白激酶是潜在的信号调节因子。在这里，我们认为番茄受体成员形成异构体复合体，以微调信号输出到下游途径，这类似于拟南芥系统，但似乎部分发散。
Ethylene receptors function in heteromeric complexes across subfamilies in tomato fruit. This finding provides insights into the regulation of ethylene signaling during tomato fruit ripening.Ripening of climacteric fruits is initiated when the gaseous plant hormone ethylene is perceived by the cell. Ethylene binding to membrane-associated ethylene receptors (ETRs) triggers a series of biochemical events through multiple components, resulting in the induction of numerous ripening-related genes. In tomato (Solanum lycopersicum L.), there are seven members of the ETR family, which each contribute to the regulation of fruit ripening. However, the relative contribution of each individual receptor to ethylene signaling remains unknown. Here, we demonstrated the formation of heteromeric receptor complexes across the two ETR subfamilies in tomato fruit. Immunoprecipitation of subfamily II SlETR4 resulted in co-purification of subfamily I (SlETR1, SlETR2, and SlETR3), but not subfamily II members (SlETR5, SlETR6, and SlETR7). Such biased interactions were verified in yeast two-hybrid assays, and in transgenic Arabidopsis plants, in which heterologous SlETR4 interacts with subfamily I ETRs. Our analysis also revealed that the receptor complexes engage the Raf-like protein kinases SlCTR1 and SlCTR3, which are potential regulators of signaling. Here, we suggest that tomato receptor members form heteromeric complexes to fine-tune signal output to the downstream pathway, which is similar to that of the Arabidopsis system but appears to be partially diverged.