Peste des petits ruminants virus detected in tissues from an Asiatic lion (Panthera leo persica) belongs to Asian lineage IV
Peste des petits ruminants virus detected in tissues from an Asiatic lion (Panthera leo persica) belongs to Asian lineage IV
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DOI：
10.4142/jvs.2012.13.2.203
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发表时间：
2012-06-01
影响因子：
1.8
通讯作者：
Singh, Raj Kumar
中科院分区：
文献类型：
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作者：
Balamurugan, Vinayagamurthy;Sen, Arnab;Singh, Raj Kumar
在这项研究中，小反刍兽疫病毒（PPRV）的冷冻混合组织样品中检测到死亡的亚洲狮（Panthera leo persica）。当使用适当的病毒特异性引物进行一步RT-PCR检测时，样本的犬瘟热病毒呈阴性，而PPRV核酸呈阳性。随后的扩增，克隆和部分核衣壳，基质和融合基因的测序证实了PPRV核酸的存在。分离病毒结构基因的比较序列和系统发育分析证实，该病毒属于亚洲谱系IV，并与印度流行的PPRV密切相关。
In this study, peste des petits ruminants virus (PPRV) was detected in frozen pooled tissue samples from a dead Asiatic lion (Panthera leo persica). The samples were negative for canine distemper virus and positive for PPRV nucleic acids when tested with one-step RT-PCR using the appropriate virus-specific primers. Subsequent amplification, cloning, and sequencing of the partial nucleocapsid, matrix, and fusion genes confirmed the presence of PPRV nucleic acid. Comparative sequence and phylogenetic analyses of the structural genes of the isolated virus confirmed that the virus belonged to Asian lineage IV and was closely related to PPRV circulating in India.