MicroRNA (miRNA) cloning analysis reveals sex differences in miRNA expression profiles between adult mouse testis and ovary
MicroRNA (miRNA) cloning analysis reveals sex differences in miRNA expression profiles between adult mouse testis and ovary
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DOI：
10.1530/rep-08-0349
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发表时间：
2008-12-01
期刊：
影响因子：
3.8
通讯作者：
Takizawa, Toshihiro
中科院分区：
文献类型：
--
作者：
Mishima, Takuya;Takizawa, Takami;Takizawa, Toshihiro
microRNAs（miRNAs）是一类内源性非编码小分子RNA，可调控互补mRNA靶点的表达。鉴定组织特异性miRNAs是理解miRNAs生物学功能的第一步，其中包括组织分化的调节和组织特性的维持。在这项研究中，我们在成年小鼠睾丸和卵巢中进行了小RNA文库测序，以揭示其特征性的器官和性别特异性的配置文件，并阐明在生殖系统中表达的miRNA的特征。从小鼠睾丸和卵巢中分别获得10852和11744个小RNA克隆（每个器官超过10000个克隆），其中已知miRNAs 6630个（159个基因）和10192个（154个基因）。结果表明，成年小鼠睾丸和卵巢的miRNA文库测序效率分别为61%（6630个miRNA克隆/10852个小RNA克隆）和87%（10192个/11744个小RNA克隆）。我们在睾丸和卵巢中获得了特征性的miRNA标签;在成年小鼠睾丸和卵巢中检测到55种高度、专门或主要的miRNA，并发现了两种新的miRNA。X染色体上出现了miRNAs的男性偏好表达。我们的数据提供了重要的信息，性别差异的miRNA表达，应促进生殖器官特异性作用的miRNA的研究。
MicroRNAs (miRNAs) are endogenous non-coding small RNAs that can regulate the expression of complementary mRNA targets. identifying tissue-specific miRNAs is the first step toward understanding the biological functions of miRNAs, which include the regulation of tissue differentiation and the maintenance of tissue identity. In this study, we performed small RNA library sequencing in adult mouse testis and ovary to reveal their characteristic organ- and gender-specific profiles and to elucidate the characteristics of the miRNAs expressed in the reproductive system. We obtained 10 852 and 11 744 small RNA clones from mouse testis and ovary respectively (greater than 10 000 clones per organ), which included 6630 (159 genes) and 10 192 (154 genes) known miRNAs. A high level of efficiency of miRNA library sequencing was achieved: 61% (6630 miRNA clones/10 852 small RNA clones) and 87% (10 192/11 744) for adult mouse testis and ovary respectively. We obtained characteristic miRNA signatures in testis and ovary; 55 miRNAs were detected highly, exclusively, or predominantly in adult mouse testis and ovary, and discovered two novel miRNAs. Male-biased expression of miRNAs occurred on the X-chromosome. Our data provide important information on sex differences in miRNA expression that should facilitate studies of the reproductive organ-specific roles of miRNAs.