Ferrigenium kumadai gen. nov., sp. nov., a microaerophilic iron-oxidizing bacterium isolated from a paddy field soi
Ferrigenium kumadai gen. nov., sp. nov., a microaerophilic iron-oxidizing bacterium isolated from a paddy field soi
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Ferrigenium kumadai gen.
DOI：
10.1099/ijsem.0.002882
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Khalifa;A.;Nakasuji;Y.;Saka;N.;Honjo;H.;Asakawa;S.;and Watanabe;T.
从日本安城县稻田土壤中分离到一株铁氧化细菌，命名为An 22 T。菌株An 22 T通过能够在12-37 °C（最佳在25-30 °C）和pH 5.2-6.8（pH 5.9-6.1）下生长的单极鞭毛、弯曲杆、革兰氏阴性细菌运动。该菌株生长微需氧和自养氧化亚铁，但没有形成秸秆，一种独特的结构的铁氧化物。细胞脂肪酸主要为C16：0和C16：1ω 7 c/C16：1ω6c。 主要呼吸醌类为UQ-10和UQ-8。该菌株具有核酮糖-1，5-二磷酸羧化酶/加氧酶，表明通过Calvin-Benson-Bassham循环的自养性质。总DNA G+C含量为61.4mol%。16 S rRNA基因序列分析表明，菌株An 22 T属于β-变形菌纲，与铁氧化细菌Gallionella ferruginea Johan（94.8%）和铁氧化细菌Ferriphaselus amnicolaOYT 1 T（94.4%）聚为一类。 基于16 S rRNA基因序列与遗传学上最接近的属的低相似性以及独特的形态、生理和生化特征的组合，菌株An 22 T代表了Gallionellaceae中的一个新属和新种，其名称为Ferrergenium kumadaigen。十一月，sp. nov.的报道。模式菌株为An 22 T（=JCM 30584 T =NBRC 112974 T =ATCC TSD-51 T）。
An iron-oxidizing bacterium, designated strain An22T, which was isolated from a paddy field soil in Anjo, Japan, was described taxonomically. Strain An22Twas motile by a single polar flagellum, curved-rod, Gram-negative bacterium that was able to grow at 12–37 °C (optimally at 25–30 °C) and at pH 5.2–6.8 (pH 5.9–6.1). The strain grew microaerobically and autotrophically by oxidizing ferrous iron, but did not form stalks, a unique structure of iron oxides. The major cellular fatty acids were C16 : 0and C16 : 1ω7c/C16 : 1ω6c. The major respiratory quinones were UQ-10 and UQ-8. The strain possessed ribulose-1,5-bisphosphate carboxylase/oxygenase indicating an autotrophic nature via the Calvin–Benson–Bassham cycle. The total DNA G+C content was 61.4 mol%. 16S rRNA gene sequence analysis revealed that strain An22Twas affiliated with the classBetaproteobacteriaand clustered with iron-oxidizing bacteria,Gallionella ferrugineaJohan (94.8 % similarity) andFerriphaselus amnicolaOYT1T(94.4 %) in the familyGallionellaceae. Based on the low 16S rRNA gene sequence similarity to the phylogenetically closest genera and the combination of unique morphological, physiological and biochemical characteristics, strain An22Trepresents a novel genus and species within the familyGallionellaceae, for which the nameFerrigenium kumadaigen. nov., sp. nov. is proposed. The type strain is An22T(=JCM 30584T=NBRC 112974T=ATCC TSD-51T).