Comparative Proteomic Analysis of Listeria monocytogenes Strains F2365 and EGD
Comparative Proteomic Analysis of Listeria monocytogenes Strains F2365 and EGD
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DOI：
10.1128/aem.01847-08
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发表时间：
2009-01-15
影响因子：
4.4
通讯作者：
Lawrence, Mark L.
中科院分区：
文献类型：
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作者：
Donaldson, Janet R.;Nanduri, Bindu;Lawrence, Mark L.
单核细胞增生李斯特菌是一种革兰氏阳性，食源性病原体，导致人类和动物的疾病。L.单核细胞增多症和13个血清型。为了进一步了解不同遗传谱系/血清型之间存在的差异。为了检测单核细胞增生，我们分析了血清型1/2a菌株EGD和血清型4 b菌株F2365在37 ℃下早期稳定期生长期间的总体蛋白表达。使用多维蛋白质鉴定技术和电喷雾串联质谱，我们从EGD和F2365中分别鉴定了1，754和1，427个蛋白质，其中1，077个是两者共有的。对菌株间表达显著改变的蛋白质的分析揭示了这两种L.单核细胞增生菌株。特别是，这些菌株在参与细胞壁生理学和鞭毛生物合成的蛋白质以及DNA修复蛋白质和应激反应蛋白质的表达方面存在差异。
Listeria monocytogenes is a gram-positive, food-borne pathogen that causes disease in both humans and animals. There are three major genetic lineages of L. monocytogenes and 13 serovars. To further our understanding of the differences that exist between different genetic lineages/serovars of L. monocytogenes, we analyzed the global protein expression of the serotype 1/2a strain EGD and the serotype 4b strain F2365 during early-stationary-phase growth at 37 C. Using multidimensional protein identification technology with electrospray ionization tandem mass spectrometry, we identified 1,754 proteins from EGD and 1,427 proteins from F2365, of which 1,077 were common to both. Analysis of proteins that had significantly altered expression between strains revealed potential biological differences between these two L. monocytogenes strains. In particular, the strains differed in expression of proteins involved in cell wall physiology and flagellar biosynthesis, as well as DNA repair proteins and stress response proteins.