Isolation and characterization of outer and inner envelope membranes of cyanelles from a glaucocystophyte, Cyanophora paradoxa
Isolation and characterization of outer and inner envelope membranes of cyanelles from a glaucocystophyte, Cyanophora paradoxa
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蓝藻藻蓝藻外膜和内膜的分离和表征
DOI：
10.1007/s11120-007-9156-2
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发表时间：
2007
影响因子：
3.7
通讯作者：
K. Satoh
中科院分区：
文献类型：
--
作者：
H. Koike;Yohei Ikeda;F. Yusa;Y. Kashino;K. Satoh
采用蔗糖密度梯度漂浮离心法从奇异蓝绿藻（Cyanophora paradoxa）中分离出囊膜。离心40 h后分离出两条黄色条带。两个组分之一（组分Y2）的浮力密度与菠菜的内被膜或蓝藻的质膜的浮力密度一致。另一个黄色级分（级分Y1）甚至在0%蔗糖下也迁移到蔗糖梯度的顶部。色素分析表明，重黄色级分富含玉米黄质，而轻级分富含β-胡萝卜素，并且两个级分几乎不含叶绿素。从藻胆蛋白级分中分离出另一个黄色级分（级分Y3），这是放置样品进行梯度离心的位置。其浮力密度和吸收光谱与蓝藻的外膜相似。我们已经分配部分Y2和Y3的内，外被膜部分的蓝藻，分别。两种包膜膜之间的蛋白质组合物是相当不同的，表明馏分之间的交叉污染很小。
Envelope membranes were isolated by sucrose density gradient floatation centrifugation from the homogenate of cyanelles prepared from Cyanophora paradoxa. Two yellow bands were separated after 40 h of centrifugation. The buoyant density of one of the two fractions (fraction Y2) coincided with that of inner envelope membranes of spinach or plasma membranes of cyanobacteria. The other yellow fraction (fraction Y1) migrated to top of sucrose-gradient even at 0% sucrose. Pigment analysis revealed that the heavy yellow fraction was rich in zeaxanthin while the light fraction was rich in β-carotene, and the both fractions contained practically no chlorophylls. Another yellow fraction (fraction Y3) was isolated from the phycobiliprotein fraction, which was the position where the sample was placed for gradient centrifugation. Its buoyant density and absorption spectra were similar to outer membranes of cyanobacteria. We have assigned fractions Y2 and Y3 as inner and outer envelope membrane fractions of cyanelles, respectively. Protein compositions were rather different between the two envelope membranes indicating little cross-contamination among the fractions.