Arabinofuranosyl nucleotides are not chain-terminators during initiation of new strands of DNA by DNA polymerase alpha-primase.
Arabinofuranosyl nucleotides are not chain-terminators during initiation of new strands of DNA by DNA polymerase alpha-primase.
复制标题
在 DNA 聚合酶 α-引物酶引发新 DNA 链的过程中，阿拉伯呋喃糖基核苷酸不是链终止子。
DOI：
10.1021/bi00035a027
复制
发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
R. Kuchta
中科院分区：
文献类型：
--
作者：
H. C. Thompson;R. Kuchta
Polymerization of NTPs and arabinofuranosyladenosine triphosphate (araATP) during DNA polymerase alpha catalyzed elongation of primase-synthesized primers was examined. After primase synthesizes a primer, pol alpha normally polymerizes multiple dNTPs onto this primer. In the absence of a required dNTP, however, primers were still elongated by up to 35 nucleotides via polymerization of the corresponding NTP in place of the missing dNTP. During the elongation of exogenously added primer/templates, however, NTPs were not readily polymerized. AraATP was readily incorporated into products during elongation of primase-synthesized primers. Importantly, polymerization of araATP did not result in chain termination; rather, the next correct nucleotide was added such that araATP was simply an alternate substrate. In contrast, polymerization of araATP during elongation of exogenously added primer/templates resulted in strong chain termination. Thus, elongation of primase-synthesized primers by pol alpha-primase is fundamentally different than elongation of exogenously added primer/templates with respect to interactions with dNTP analogs. Furthermore, these data provide a rationale for how araNMPs are efficiently incorporated into internucleotide linkages of DNA in whole cells and suggest that the initiation of new strands of DNA by pol alpha-primase may be a unique target for inhibiting replication.
单个哺乳动物引物酶亚基的酶学表征揭示了 DNA 复制起始的双相机制。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Copeland,WC;Wang,TS
通讯作者：
Wang,TS
影响因子：
14.9
作者：
MILLIGAN, JF;GROEBE, DR;UHLENBECK, OC
通讯作者：
UHLENBECK, OC
来自 KB 细胞的 DNA 引物酶。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hu,SZ;Wang,TS;Korn,D
通讯作者：
Korn,D