CLONING OF THE CDNA OF THE HEME-REGULATED EUKARYOTIC INITIATION FACTOR-2-ALPHA (EIF-2-ALPHA) KINASE OF RABBIT RETICULOCYTES - HOMOLOGY TO YEAST GCN2 PROTEIN-KINASE AND HUMAN DOUBLE-STRANDED-RNA-DEPENDENT EIF-2-ALPHA KINASE
CLONING OF THE CDNA OF THE HEME-REGULATED EUKARYOTIC INITIATION FACTOR-2-ALPHA (EIF-2-ALPHA) KINASE OF RABBIT RETICULOCYTES - HOMOLOGY TO YEAST GCN2 PROTEIN-KINASE AND HUMAN DOUBLE-STRANDED-RNA-DEPENDENT EIF-2-ALPHA KINASE
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DOI：
10.1073/pnas.88.17.7729
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发表时间：
1991-09-01
影响因子：
11.1
通讯作者：
LONDON, IM
中科院分区：
文献类型：
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作者：
CHEN, JJ;THROOP, MS;LONDON, IM
我们克隆了兔网织红细胞血红素调节的eIF-2-alpha激酶（HRI）的cDNA。从HRI cDNA转录的mRNA的体外翻译产生90-kDa多肽，其表现出eIF-2-α激酶活性，并被针对真实HRI的单克隆抗体识别。HRI cDNA的开放阅读框序列包含蛋白激酶的所有11个催化结构域，在保守的催化结构域VI和VIII中具有蛋白丝氨酸/苏氨酸激酶的共有序列。HRI cDNA在催化结构域V和VI之间还含有几乎等于140个氨基酸的插入物。HRI cDNA编码序列与酿酒酵母的GCN 2蛋白激酶和人双链RNA依赖性eIF-2-alpha激酶具有广泛的同源性。这一观察结果表明，GCN 2蛋白激酶可能是酵母中的eIF-2-α激酶。此外，HRI与参与细胞周期调节的三种蛋白激酶（NimA、Weel和CDC 2）具有异常高的同源性。
We have cloned the cDNA of the heme-regulated eIF-2-alpha kinase (HRI) of rabbit reticulocytes. In vitro translation of mRNA transcribed from the HRI cDNA yields a 90-kDa polypeptide that exhibits eIF-2-alpha kinase activity and is recognized by a monoclonal antibody directed against authentic HRI. The open reading frame sequence of the HRI cDNA contains all 11 catalytic domains of protein kinases with consensus sequences of protein-serine/threonine kinases in conserved catalytic domains VI and VIII. The HRI cDNA also contains an insert of almost-equal-to 140 amino acids between catalytic domains V and VI. The HRI cDNA coding sequence has extensive homology to GCN2 protein kinase of Saccharomyces cerevisiae and to human double-stranded-RNA-dependent eIF-2-alpha kinase. This observation suggests that GCN2 protein kinase may be an eIF-2-alpha kinase in yeast. In addition, HRI has an unusually high degree of homology to three protein kinases (NimA, Weel, and CDC2) that are involved in the regulation of the cell cycle.