Adipose and Liver Expression of Interleukin (IL)-1 Family Members in Morbid Obesity and Effects of Weight Loss
Adipose and Liver Expression of Interleukin (IL)-1 Family Members in Morbid Obesity and Effects of Weight Loss
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DOI：
10.2119/molmed.2010.00108
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发表时间：
2011-07-01
影响因子：
5.7
通讯作者：
Tilg, Herbert
中科院分区：
文献类型：
--
作者：
Moschen, Alexander R.;Molnar, Clemens;Tilg, Herbert
病态肥胖与慢性炎症状态有关。白细胞介素-1家族（IL-1F）细胞因子成员是由肥胖的人类脂肪组织产生的。虽然某些IL-1F成员如IL-1 β或IL-18具有强效促炎作用，但其他成员如IL-1受体拮抗剂（IL-1Ra）或IL-37（以前称为IL-1F7）具有抗炎作用。NLRP3炎症小体在生物活性IL-1 β和IL-18的加工过程中起关键作用。我们研究了过度减肥对皮下脂肪组织和肝脏IL-1 α、IL-1 β、IL-18、IL-1Ra、IL-37和NLRP3表达的影响。对21例重度肥胖患者行腹腔镜胃束带术进行了研究。在腹腔镜可调节胃束带手术前和术后6个月采集组织样本。所有研究的IL-1F成员的mRNA表达，尤其是IL-37，在皮下/内脏脂肪组织中的表达比在肝脏中的表达要高得多。广泛减肥后皮下脂肪组织IL-1 β mRNA表达显著降低；IL-18和IL-1Ra的表达没有变化，而IL-37的表达增加。体重减轻导致肝脏IL-1 β、IL-18和IL-1Ra表达显著降低，而肝脏IL-37 mRNA表达保持稳定。脂肪/肝脏NLRP3炎性体和il - 1 α的表达不受体重减轻的影响。组织中IL-1 β、IL-18和IL-37在皮下/内脏脂肪组织中的表达明显高于肝脏。综上所述，在重度肥胖患者中，IL-1F成员在脂肪组织中的表达比在肝组织中的表达更为明显。过度减肥使IL-1F成员的脂肪和肝脏表达谱向更抗炎的方向变化。(C) 2011年，范斯坦医学研究所，www.feinsteininstitute.org
Morbid obesity is associated with a state of chronic inflammation. Interleukin-1 family (IL-1F) cytokine members are produced by human adipose tissue in obesity. Whereas certain IL-1F members such as IL-1 beta or IL-18 are potently proinflammatory, others such as IL-1 receptor antagonist (IL-1Ra) or IL-37 (formerly IL-1F7) are antiinflammatory. The NLRP3 inflammasome plays a key role in the processing of bioactive IL-1 beta and IL-18. We investigated the effect of excessive weight loss on subcutaneous adipose tissue and liver expression of IL-1 alpha, IL-1 beta, IL-18, IL-1Ra, IL-37 and NLRP3. Twenty-one severely obese patients undergoing laparoscopic adjustable gastric banding were studied. Tissue samples were collected before and 6 months after laparoscopic adjustable gastric banding surgery. mRNA expression of all studied IL-1F members, but especially of IL-37, was much higher in subcutaneous/visceral adipose tissue compared with their liver expression. Subcutaneous adipose tissue mRNA expression of IL-1 beta decreased significantly after extensive weight loss; expression of IL-18 and IL-1Ra did not change, whereas IL-37 expression increased. Weight loss led to a significant reduction in liver IL-1 beta, IL-18 and IL-1Ra expression, whereas hepatic IL-37 mRNA expression remained stable. Adipose/liver NLRP3 inflammasome and IL-l alpha expression were not affected by weight loss. Tissue expression of IL-1 beta, IL-18 and IL-37 were significantly higher in subcutaneous/visceral adipose tissue compared with the liver. In conclusion, expression of IL-1F members is more pronounced in adipose compared with liver tissue in patients with severe obesity. Excessive weight loss changes the adipose and liver expression profile of IL-1F members toward a more antiinflammatory direction. (C) 2011 The Feinstein Institute for Medical Research, www.feinsteininstitute.org