Rapid determination of flavonoids in plumules of sacred lotus cultivars and assessment of their antioxidant activities
Rapid determination of flavonoids in plumules of sacred lotus cultivars and assessment of their antioxidant activities
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神莲品种胚芽中黄酮类化合物的快速测定及其抗氧化活性评估
DOI：
10.1016/j.indcrop.2016.04.030
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发表时间：
2016-09-01
影响因子：
5.9
通讯作者：
Wang, Liang-Sheng
中科院分区：
文献类型：
--
作者：
Feng, Cheng-Yong;Li, Shan-Shan;Wang, Liang-Sheng
据《中国药典》记载，莲子心可入药。本研究首次采用超高效液相色谱-光电二极管阵列（UPLC-PDA）和超高效液相色谱-串联质谱（UPLC-MS/MS）对38个莲藕品种幼芽中的黄酮类化合物进行了定量分析。共检测并鉴定了20种黄酮类化合物，其中14种为黄酮C-糖苷。这些品种被聚成三个聚类特征的总和检测黄酮类化合物。此外，我们的研究表明，总多酚含量与DPPH法和FRAP法测定的抗氧化活性呈显著正相关。其中"太空莲"、"金七"、"银球"和"红太莲"具有较强的抗氧化活性，可用于保健品的生产。本研究对更全面地了解莲子心的药用价值具有重要意义。同时，也为莲心的商品化开发提供了参考。(C)© 2016 Elsevier B.V.版权所有。
As recorded in "Chinese Pharmacopoeia", lotus plumule can be used as medicine. In present study, flavonoids in plumules of 38 lotus (Nelumbo nucifera Gaertner) cultivars were evaluated and quantified by ultra-performance liquid chromatography with photodiode array (UPLC-PDA) and ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) for the first time. In total, twenty flavonoids were detected and identified, among which fourteen were found to be flavonoid C-glycosides. These cultivars were clustered into three clusters characterized by sum of detected flavonoids. Furthermore, our study showed that the correlation between total polyphenol content and antioxidant activity which was measured by DPPH and FRAP assays was significant positive. Among them, 'Taikonglian', 'Jinqi', 'Yinqiu', and 'Hongtailian' exhibited the greater potential of antioxidant activity and could be utilized in the production of healthcare products. This research is of great value to more comprehensive understanding of medicinal property of lotus plumule. Also, it will be helpful in the development of commercial exploitation of lotus plumule. (C) 2016 Elsevier B.V. All rights reserved.