LIGHT-DEPENDENT FIXATION OF POLYAMINES INTO CHLOROPLASTS OF CHINESE CABBAGE
LIGHT-DEPENDENT FIXATION OF POLYAMINES INTO CHLOROPLASTS OF CHINESE CABBAGE
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DOI：
10.1016/0014-5793(82)80023-9
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发表时间：
1982-01-01
期刊：
影响因子：
3.5
通讯作者：
BALINT, RF
中科院分区：
文献类型：
--
作者：
COHEN, SS;MARCU, DE;BALINT, RF
我们一直在研究芜菁黄花叶病毒（TYMV）对大白菜的感染，TYMV是一种引起叶绿体膜泡化、叶绿体聚集和病毒积累的感染，特别是在叶绿体之间的口袋中[1，2]。TYMV富含与RNA相关的亚精胺[3]，受感染的植物含有高水平的这种三胺[4]。我们已经观察到从叶原生质体分离的叶绿体组分可以由甲硫氨酸合成亚精胺，并且含有亚精胺合酶[S]。受感染的植物也富含腐胺和精胺，前者在感染后期增加，显然伴随着植物的黄化和枯萎[4]。由于已知腐胺通常在不利土壤中的植物中积累[6]，并且本身对植物有毒[7]，我们已经询问了这种多胺在感染中的增加是否与发展中的病理学有因果关系。关于这一点，有两组意见：(1)腐胺被发现在光照下对蓝细菌Anacystis nidulans是致命的，并且与各种细胞蛋白质共价结合，包括核糖体亚基[81;（2）健康叶片的原生质体被发现通过从破裂的原生质体分离的叶绿体将甲硫氨酸转化为酸不溶性形式。已经发现，在光的存在下，叶绿体确实以酸不溶性形式结合多胺，其中约1/2可溶于80%丙酮或乙醇。
We have been studying the infection of Chinese cabbage by turnip yellow mosaic virus (TYMV), an infection which causes vesiculation of chloroplast membranes, aggregation of chloroplasts and accumulation of virus specifically in pockets between the chloroplasts [1, 2]. TYMV is rich in spermidine associated with the RNA [3] and the infected plant contains high levels of this triamine [4]. We have observed that a chloroplast fraction isolated from leaf protoplasts can synthesize spermidine from methionine and contains spermidine synthase [S]. The infected plant is also high in putrescine and spermine, and the former increases late in infection, apparently concomitantly with the yellowing and withering of the plant [4]. Since it is known that putrescine accumulates generally in plants in unfavorable soils [6] and is itself toxic to the plant [7], we have asked if the increase of this polyamine in infection is causally related to the developing pathology. Two sets of observations bear on this point:.(1) Putrescine was found to be lethal to a Cyanobacterium, Anacystis nidulans in the light, and to bind covalently to various cellular proteins, including ribosomal subunits [81;(2) Protoplasts of the healthy leaves were found to convert methionine to acid-insoluble form by chloroplasts isolated from disrupted protoplasts. It has been found that in the presence of light, chloroplasts do bind polyamines in an acid-insoluble form, of which-l/2 is soluble in 80% acetone or alcohol.