Effects of Teriparatide on Bone in Autochthonous Transgenic Model Mice for Diabetes Mellitus (Akita Mice)
Effects of Teriparatide on Bone in Autochthonous Transgenic Model Mice for Diabetes Mellitus (Akita Mice)
复制标题
特立帕肽对糖尿病转基因模型小鼠（秋田小鼠）骨的影响
DOI：
10.1016/j.afos.2019.11.003
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Shimada Y.
中科院分区：
文献类型：
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作者：
Ohuchi K;Miyakoshi N;Kasukawa Y;Segawa T;Kinoshita H;Sato C;Fujii M;Shimada Y.
目的本研究的目的是评价特立帕肽（TPTD）对糖尿病秋田小鼠模型的骨密度（BMD）、骨强度和骨质量的影响。方法将12周龄雌性秋田小鼠和对照小鼠（C57/BL/6NCrSlc）分为4组：对照组小鼠用媒介物治疗（n = 7）或TPTD治疗（n = 6）；和用媒介物 (n = 6) 或 TPTD (n = 7) 治疗的秋田小鼠。 TPTD或媒介物每周皮下注射3次，持续8周。采用傅里叶变换红外光谱成像技术评估血糖、血清硬化素、胫骨总骨密度、股骨干骨强度和骨质量。结果治疗8周后，各组血清硬化素水平没有明显差异。与载体相比，TPTD 显着增加了对照小鼠（+12.7%，P = 0.02）和秋田小鼠（+29.2%，P = 0.001）的 BMD。 TPTD 治疗的秋田小鼠的最大负载和刚度显着高于载体治疗的秋田小鼠（分别为 +56.6%，P = 0.03 和 + 90.5%，P = 0.02）。在傅里叶变换红外光谱成像中，用赋形剂治疗的秋田小鼠的矿物质/基质比率显着低于对照小鼠（-12.2%，P = 0.02），并且TPTD治疗显着增加了矿物质/基质比率（P = 0.003）。结论TPTD因此改善了对照小鼠和秋田小鼠的BMD和骨强度，并且改善了秋田小鼠的矿物质/基质比率。
ObjectivesThe purpose of this study is to evaluate the effects of teriparatide (TPTD) on bone mineral density (BMD), bone strength, and bone quality in Akita mouse models of diabetes mellitus.MethodsTwelve-week-old female Akita mice and control mice (C57/BL/6NCrSlc) were divided into 4 groups: control mice treated with vehicle (n = 7) or TPTD (n = 6); and Akita mice treated with vehicle (n = 6) or TPTD (n = 7). TPTD or vehicle was administered subcutaneously 3 times a week for 8 weeks. Blood glucose, serum sclerostin, total tibial BMD, femoral shaft bone strength, and bone quality using Fourier-transform infrared spectroscopy imaging were evaluated.ResultsNo significant differences in serum sclerostin levels were evident among these groups after 8 weeks of treatment. TPTD significantly increased BMD in control mice (+12.7%, P = 0.02) and Akita mice (+29.2%, P = 0.001) compared with vehicle. Maximum load and stiffness were significantly higher in Akita mice treated with TPTD than in Akita mice treated with vehicle (+56.6%, P = 0.03 and + 90.5%, P = 0.02, respectively). On Fourier-transform infrared spectroscopy imaging, the mineral/matrix ratio was significantly lower in Akita mice treated with vehicle than in control mice (−12.2%, P = 0.02), and TPTD treatment significantly increased the mineral/matrix ratio (P = 0.003).ConclusionsTPTD thus improved BMD and bone strength in both control mice and Akita mice, with improvements in the mineral/matrix ratio among Akita mice.