An in vitro study of the properties of vagal afferent fibres innervating the ferret oesophagus and stomach
An in vitro study of the properties of vagal afferent fibres innervating the ferret oesophagus and stomach
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DOI：
10.1111/j.1469-7793.1998.907bd.x
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发表时间：
1998-11-01
影响因子：
5.5
通讯作者：
Blackshaw, LA
中科院分区：
文献类型：
--
作者：
Page, AJ;Blackshaw, LA
1.采用体外培养的带迷走神经的食道标本，研究了单个迷走神经传入纤维的特性。记录来自三种类型的胃-食道迷走神经传入纤维，根据它们对机械刺激的敏感性进行分类。有对黏膜敲击有反应的受体(粘膜受体)，对环形张力有反应的(张力受体)，也有对粘膜敲击和环形张力有反应的受体，我们称之为张力/粘膜(TM)受体。黏膜、TM和张力感受器纤维的传导速度分别为6.38+/-1.22m S(-1)(n=22)、6.20+/-1.49m S(-1)(n=13)和5.33+/-0.86m S(-1)(n=22)。传入神经元的感受野按纤维类型和传导速度呈随机的地形图分布。它们主要在迷走神经剥离点的远端被发现，但偶尔也被发现在近端。28%的粘膜、63%的TM和43%的张力感受器对一种或多种药物或化学刺激应用于感受野有反应。综上所述，本实验为食道迷走神经传入纤维存在三种类型提供了证据，即粘膜、张力和新近发现的张力/粘膜受体。
1. A novel preparation of the oesophagus with attached vagus nerve from the ferret maintained in vitro was used to study the properties of single vagal afferent nerve fibres with identified receptive fields.2. Recordings were made from three types of gastro-oesophageal vagal afferent fibres that were classified on the basis of their sensitivity to mechanical stimulation. There were those responding to mucosal stroking (mucosal receptors), to circular tension (tension receptors) and those responding to mucosal stroking and circular tension, which we have termed tension/mucosal (TM) receptors.3. The conduction velocities for mucosal, TM and tension receptor fibres were 6.38 +/- 1.22 m s(-1) (n = 22), 6.20 +/- 1.49 m s(-1) (n = 13) and 5.33 +/- 0.86 m s(-1) (n = 22), respectively.4. Receptive fields of afferents showed random topographical distribution by fibre type and conduction velocity. They were found mainly distal but also occasionally proximal to the point of vagal dissection.5. Twenty-eight per cent of mucosal, 63% of TM and 43% of tension receptors responded to one or more drugs or chemical stimuli applied to the receptive field.6. In conclusion, this experimental preparation provides evidence for the existence of three types of oesophageal vagal afferent fibre, namely mucosal, tension and the newly identified tension/mucosal receptors.