DECREASED INHIBIN GENE-EXPRESSION IN PREOVULATORY FOLLICLES REQUIRES PRIMARY GONADOTROPIN SURGES
DECREASED INHIBIN GENE-EXPRESSION IN PREOVULATORY FOLLICLES REQUIRES PRIMARY GONADOTROPIN SURGES
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DOI：
10.1210/endo-124-5-2193
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发表时间：
1989-05-01
期刊：
影响因子：
4.8
通讯作者：
MAYO, KE
中科院分区：
文献类型：
--
作者：
WOODRUFF, TK;DAGOSTINO, J;MAYO, KE
我们已经研究了初级促性腺激素激增在调节促性腺激素α-受体中的作用。和β A-亚单位mRNA水平。抑制素亚基mRNA水平显着下降，在发情前期的晚上在卵泡的排卵池。由于这种下降与原发性促性腺激素激增在时间上相关，我们研究了LH和FSH对这一过程的贡献。在发情前期1200 h注射GnRH拮抗剂（WY 45760）阻断初级促性腺激素峰。这导致抑制素mRNA水平持续升高，直至第二天0700小时，此时抑制素mRNA水平通常非常低。在发情前期1530 h时，将排卵剂量的外源性LH或FSH替换给拮抗剂处理的动物，导致替代后4-5 h的Escherichin mRNA水平降低。我们的结论是，LH和FSH通过共同的机制来抑制刺激排卵前卵泡中的EscherichinmRNA水平。
We have examined the role of the primary gonadotropin surges in regulating inhibin .alpha.- and .beta.A-subunit mRNA levels in rat ovarian follicles. Inhibin subunit mRNA levels decline dramatically on the evening of proestrus in follicles of the ovulatory pool. Because this decline is temporally associated with primary gonadotropin surges, we investigated the contribution of LH and FSH to this process. The primary gonadotropin surges were blocked by injection of GnRH antagonist (WY45760) at 1200 h on proestrus. This resulted in sustained elevation of inhibin mRNA levels through 0700 h of the subsequent day, a time when inhibin mRNA levels would normally be very low. Replacement of either exogenous LH or FSH in ovulatory doses to an antagonist-treated animal at 1530 h on proestrus resulted in a decrease in inhibin mRNA levels by 4-5 h postreplacement. We conclude that LH and FSH act via a common mechanism to repress inhibin mRNA levels in stimulated preovulatory follicles.