Enhanced cementum formation in experimentally induced cementum defects of the root surface with the application of recombinant basic fibroblast growth factor in collagen gel in vivo
Enhanced cementum formation in experimentally induced cementum defects of the root surface with the application of recombinant basic fibroblast growth factor in collagen gel in vivo
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DOI：
10.1902/jop.2004.75.2.243
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发表时间：
2004-02-01
影响因子：
4.3
通讯作者：
Kuboki, Y
中科院分区：
文献类型：
--
作者：
Sato, Y;Kikuchi, M;Kuboki, Y
背景：使用具有生物活性的、可溶的因子，如生长因子或细胞因子，已被研究用于修复牙周炎引起的牙周组织的潜在临床应用。碱性成纤维细胞生长因子是一种多功能的生长因子，在包括牙周韧带组织在内的多种细胞和组织中具有诱导增殖和形态发生的作用。方法：在实验诱导的Beagle犬部分缺损模型中，观察碱性成纤维细胞生长因子对牙骨质和牙周膜再生的影响。结果：术后8周，与单纯胶原凝胶相比，0.1、1、5µg的胶原凝胶均能促进牙本质牙骨质的形成。组织学分析显示，术后4周，随机分布的牙周膜纤维已经与牙本质结合，但仅附着在裸露的牙本质上，在牙本质上应用0.1、1或5杯胶原凝胶中的碱性成纤维生长因子。术后8周，我们观察到1杯bFGFF处理的牙形成致密的纤维结合到牙槽骨和新合成的牙骨质中。结论：碱性成纤维细胞生长因子胶原凝胶是一种适合牙周膜损伤的治疗方法，为牙周病的治疗提供了一种新的方法。
Background: Therapies using biologically active, soluble factors such as growth factors or cytokines have been investigated for potential clinical use in regenerating lost periodontal tissue due to periodontitis. Basic fibroblast growth factor (bFGF, FGF-2) is a multifunctional growth factor that has a variety of effects including induction of proliferation and morphogenesis in a wide range of cells and tissues including periodontal ligament tissue.Methods: In this study, we examined the effects of bFGF on the regeneration of cementum and periodontal ligament in experimentally induced partial defects in a beagle dog model. bFGF in a Collagen gel was applied to the defects and root surfaces, and the teeth were replanted.Results: Eight weeks post-surgery, formation of cementum on denuded dentin was enhanced by application of 0.1, 1, or 5 mug of bFGF in a Collagen gel compared to Collagen gel containing vehicle. Histological analyses revealed that at 4 weeks post-surgery, random periodontal ligament fibers had bound to dentin, but were attached only to denuded dentin to which 0.1, 1, or 5 mug of bFGF in Collagen gel had been applied. At 8 weeks post-surgery, we observed the formation of dense fibers bound to alveolar bone and newly synthesized cementum in teeth treated with 1 mug of bFGF.Conclusion: These results suggest that basic fibroblast growth factor in a collagen gel is a suitable therapy for damaged periodontal ligament and could lead to readily achievable methods of treatment for periodontal disease.