Evaluation of three automated chromogenic FVIII kits for the diagnosis of mild discrepant haemophilia A
Evaluation of three automated chromogenic FVIII kits for the diagnosis of mild discrepant haemophilia A
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DOI：
10.1111/j.1751-553x.2007.01021.x
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发表时间：
2009-04-01
影响因子：
3
通讯作者：
Lloyd, J. V.
中科院分区：
文献类型：
--
作者：
Rodgers, S. E.;Duncan, E. M.;Lloyd, J. V.
在一些轻度血友病A患者（表型差异）中，通过一期测定法（FVIII-1st）测得的凝血因子VIII水平是通过经典两阶段凝血测定法（FVIII-2st）测得的凝血因子VIII水平的两倍多，并且可能在正常范围内。我们的目的是评估自动两阶段显色FVIII检测（FVIII-chr）用于诊断轻度不一致血友病A。使用推荐的和延长的孵育时间评价了三种显色FVIII试剂盒（Biophen、Coamatic和Dade-Behring）。检测了来自不一致血友病（n = 7）和等效轻度血友病（FVIII-1st和FVIII-2st之间一致，n = 4）患者的样本。对于等效血友病，在所有孵育时间，所有试剂盒的FVIII-chr均与FVIII-1st和FVIII-2st一致。对于不一致血友病，使用推荐的孵育时间，使用Biophen试剂的平均FVIII-chr为22 IU/dl（范围13-31），Coamatic为26（17-34），Dade-Behring为41（33-47），而FVIII-1st为36（27-44），FVIII-2st为8（6-9）。随着Biophen和Coamatic孵育时间的增加，FVIII-chr降低，但Dade-Behring降低较少。使用Dade-Behring试剂盒的FVIII-chr得到的结果与FVIII-1st相似，不适用于诊断轻度差异性血友病A。Biophen和Coamatic试剂盒的FVIII-chr适用于这些患者的诊断，特别是延长孵育时间。
In some mild haemophilia A patients (discrepant phenotype), coagulation FVIII levels by one-stage assay (FVIII-1st) are more than double those by classical two-stage coagulation assay (FVIII-2st), and may fall within the normal range. Our aim was to assess automated two-stage chromogenic FVIII assays (FVIII-chr) for diagnosis of mild discrepant haemophilia A. Three chromogenic FVIII kits (Biophen, Coamatic and Dade-Behring) were evaluated, using recommended and extended incubation times. Samples were tested from patients with discrepant haemophilia (n = 7) and equivalent mild haemophilia (agreement between FVIII-1st and FVIII-2st, n = 4). For equivalent haemophilia, FVIII-chr were consistent with FVIII-1st and FVIII-2st for all kits at all incubation times. For discrepant haemophilia, using recommended incubation times, mean FVIII-chr using Biophen reagents was 22 IU/dl (range 13-31), with Coamatic 26 (17-34) and with Dade-Behring 41 (33-47), compared with 36 (27-44) for FVIII-1st and 8 (6-9) for FVIII-2st. FVIII-chr decreased as incubation time was increased with Biophen and Coamatic, but decreased less with Dade-Behring. FVIII-chr using the Dade-Behring kit gave similar results to FVIII-1st and is not suitable for diagnosis of mild discrepant haemophilia A. FVIII-chr by Biophen and Coamatic kits is suitable for diagnosis of these patients, especially with an extended incubation time.