COCCOLITH‐ASSOCIATED POLYSACCHARIDES FROM CELLS OF EMILIANIA HUXLEYI (HAPTOPHYCEAE) 1
COCCOLITH‐ASSOCIATED POLYSACCHARIDES FROM CELLS OF EMILIANIA HUXLEYI (HAPTOPHYCEAE) 1
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来自 EMILIANIA HUXLEYI (HAPTOPHYCEAE) 细胞的颗石相关多糖 1
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
J. Kamerling
中科院分区：
文献类型：
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作者：
A. Borman;E. Jong;R. Thierry;P. Westbroek;L. Bosch;M. Gruter;J. Kamerling
huxleyi (Lohmann) Hay and Mohler的球粒石是一种单细胞钙化藻，由方解石和酸性Ca2+结合多糖紧密结合而成。这种多糖被认为通过干扰CaCO3结晶在球粒合成中起调节作用。本研究表明，a92、L和92 D三种不同菌株的多糖对CaCO3的体外沉淀均有相同程度的抑制作用。a92和L多糖的单糖组成相似。然而，92 D材料与其他两种材料不同：它含有的甲基化糖和核糖含量明显较低，鼠李糖和半乳糖含量较高。它还含有在a92和L多糖中未检测到的抗原决定因子。与后两种大分子不同，92 D多糖在聚丙烯酰胺凝胶电泳上以两条条带的形式迁移，这可能是由于与少量蛋白质络合的结果。从L细胞中提取的球粒多糖，在生长速度加快的情况下，也会以两种方式迁移。这种现象是由于分子大小分布的增加。结果表明，分子的某些性质可能会发生变化，但不会干扰其功能。
Coccoliths of Emiliania huxleyi (Lohmann) Hay and Mohler, a unicellular calcifying alga, consist of calcite closely associated with an acidic, Ca2+‐binding polysaccharide. This polysaccharide is thought to play a regulatory role in coccolith synthesis by interfering with CaCO3 crystallization. Here we show that the polysaccharides from three different strains, A 92, L and 92 D, all inhibit the precipitation of CaCO3 in vitro to the same extent. The monosaccharide compositions of the A 92 and L polysaccharide are similar. The 92 D material, however, deviates from the other two: it contains significantly lower amounts of methylated sugars and ribose, and elevated levels of rhamnose and galactose. It also contains antigenic determinants not detected in the A 92 and L polysaccharides. In contrast to the latter two macromolecules the 92 D polysaccharide migrates as two bands upon polyacrylamide gel electrophoresis, possibly resulting from complexing with small amounts of protein. The coccolith polysaccharide from L cells, cultured at an elevated growth rate, also migrates as two bands. This phenomenon is due to an increase in molecular size distribution. The results suggest that certain properties of the molecule may be subject to variation without interfering with its function.