Biosynthesis of glycosaminoglycans by the separated tissues of the embryonic chick cornea.
Biosynthesis of glycosaminoglycans by the separated tissues of the embryonic chick cornea.
复制标题
胚胎鸡角膜分离组织的糖胺聚糖的生物合成。
DOI：
10.1016/0012-1606(78)90094-5
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发表时间：
1978
影响因子：
2.7
通讯作者：
G. Hart
中科院分区：
文献类型：
--
作者：
G. Hart
从孵育14、17和20天的鸡胚中分离角膜组织（上皮、内皮和基质），并立即在体外用d-[6- 3 H]葡萄糖胺和H235 SO 4标记。结合凝胶过滤色谱法，通过特定降解技术测定掺入每种类型糖胺聚糖或糖肽中的标记物的量。结果表明，角膜上皮合成很少，如果有的话，硫酸角膜角质素，但角膜内皮可能合成了少量的硫酸角膜角质素。几乎所有的角膜硫酸角质素都来源于基质。角膜硫酸乙酰肝素似乎主要来源于发育后期的角膜上皮。角膜内皮细胞对角膜的透明质酸有很大的贡献。只有上皮细胞产生了很大比例的硫酸化糖蛋白。此外，上皮合成了大比例的硫酸化的高分子量多糖，其对降解已知类型的糖胺聚糖的处理具有抗性。每个角膜组织不仅可以通过向细胞外基质贡献独特的糖基化蛋白质谱来直接影响角膜形态发生，而且可以通过调节其他角膜组织的生物合成活性来间接调节细胞外基质组成。
Corneal tissues (epithelium, endothelium, and stroma) were isolated from chick embryos at 14, 17, and 20 days of incubation and immediately labeledin vitrowithd-[6-3H]glucosamine and H235SO4. Amount of label incorporated into each type of glycosaminoglycan or into glycopeptides was determined by specific degradative techniques, in conjunction with gel filtration chromatography. Results suggested that corneal epithelium synthesized little, if any, corneal keratan sulfates, but that corneal endothelium may have synthesized small amounts of corneal keratan sulfates. Nearly all corneal keratan sulfates were derived from the stroma. Corneal heparan sulfates appeared to be derived predominantly from corneal epithelium at later stages of development. Corneal endothelium contributed large proportions of the hyaluronic acids of the cornea. Only epithelium produced a large proportion of sulfated glycoproteins. In addition, epithelium synthesized a large proportion of a sulfated, high molecular weight polysaccharide which was resistant to treatments degrading known types of glycosaminoglycans. Each corneal tissue may not only affect corneal morphogenesis directly by contributing a unique spectrum of glycosylated proteins to the extracellular matrix, but also may regulate the extracellular matrix composition indirectly by modulating the biosynthetic activities of the other corneal tissues.