Characterization of the biotin biosynthesis pathway in Saccharomyces cerevisiae and evidence for a cluster containing BIO5, a novel gene involved in vitamer uptake.
Characterization of the biotin biosynthesis pathway in Saccharomyces cerevisiae and evidence for a cluster containing BIO5, a novel gene involved in vitamer uptake.
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DOI：
10.1016/s0378-1119(99)00117-1
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发表时间：
1999-05
期刊：
影响因子：
3.5
通讯作者：
V. Phalip;Isabelle Kuhn;Yves Lemoine;J. Jeltsch
中科院分区：
文献类型：
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作者：
V. Phalip;Isabelle Kuhn;Yves Lemoine;J. Jeltsch
已分离到一种影响生物素生物合成的酿酒酵母工程突变株。该突变体允许表征生物簇（BIO 3 -4-5）。我们证明，BIO 3（YNR 058 w）和BIO 4（YNR 057 c）编码，分别为7，8-二氨基壬酸氨基转移酶和脱硫生物素合成酶，参与生物素的生物合成途径。一个新的基因，BIO 5（YNR 056 c），存在于紧邻BIO 4的下游。该基因编码Bio 5 p，一种具有11个推定跨膜区的蛋白质。用标记的7-酮基8-氨基壬酸进行的摄取实验表明，Bio 5 p负责将7-酮基8-氨基壬酸转运到细胞中。
An engineered mutant of Saccharomyces cerevisiae affected in biotin biosynthesis has been isolated. This mutant allowed the characterization of a bio cluster (BIO3–4–5). We demonstrate that BIO3 (YNR058w) and BIO4 (YNR057c) encode, respectively, a 7,8-diaminopelargonic acid aminotransferase and a dethiobiotin synthase, involved in the biotin biosynthesis pathway. A novel gene, BIO5 (YNR056c), is present immediately downstream from BIO4. This gene encodes Bio5p, a protein with 11 putative transmembrane regions. Uptake experiments performed with labeled 7-keto 8-aminopelargonic acid indicate that Bio5p is responsible for transport into the cell of 7-keto 8-aminopelargonic acid.