Phylogenetic diversity of culturable bacteria from Antarctic sandy intertidal sediments
Phylogenetic diversity of culturable bacteria from Antarctic sandy intertidal sediments
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DOI：
10.1007/s00300-009-0758-3
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发表时间：
2010-06
期刊：
影响因子：
1.7
通讯作者：
Yong Yu;Huirong Li;Yin–Xin Zeng;Bo Chen
中科院分区：
文献类型：
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作者：
Yong Yu;Huirong Li;Yin–Xin Zeng;Bo Chen
研究了中国南极中山站沿岸沙质潮间带沉积物中可培养细菌的多样性。在4℃下共分离到65株需氧异养细菌，镜检和16S rRNA基因序列分析表明，这些菌株以革兰氏阴性菌为主，仅分离到16株革兰氏阳性菌。这些细菌分离株可分为5个系统发育群：α-和伽马变形杆菌、类杆菌、放线杆菌和细菌。根据系统发育树，所有65个分离物被分为29个主要类群，对应至少29个不同的属。根据序列分析(98%的序列相似性)，南极分离株至少属于37个不同的细菌物种，其中14个细菌物种(37.8%)代表了潜在的新类群。这些结果表明，南极沙质潮间带沉积物中的细菌群落具有高度的可培养多样性。
The diversity of culturable bacteria associated with sandy intertidal sediments from the coastal regions of the Chinese Antarctic Zhongshan Station on the Larsemann Hills (Princess Elizabeth Land, East Antarctica) was investigated. A total of 65 aerobic heterotrophic bacterial strains were isolated at 4°C. Microscopy and 16S rRNA gene sequence analysis indicated that the isolates were dominated by Gram-negative bacteria, while only 16 Gram-positive strains were isolated. The bacterial isolates fell in five phylogenetic groups: Alpha- and Gammaproteobacteria, Bacteroidetes, Actinobacteria and Firmicutes. Based on phylogenetic trees, all the 65 isolates were sorted into 29 main clusters, corresponding to at least 29 different genera. Based on sequence analysis (<98% sequence similarity), the Antarctic isolates belonged to at least 37 different bacterial species, and 14 of the 37 bacterial species (37.8%) represented potentially novel taxa. These results indicated a high culturable diversity within the bacterial community of the Antarctic sandy intertidal sediments.