Characterization of neurotransmitters and related metabolites in Daphnia magna juveniles deficient in serotonin and exposed to neuroactive chemicals that affect its behavior: A targeted LC-MS/MS method
Characterization of neurotransmitters and related metabolites in Daphnia magna juveniles deficient in serotonin and exposed to neuroactive chemicals that affect its behavior: A targeted LC-MS/MS method
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DOI：
10.1016/j.chemosphere.2020.127814
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发表时间：
2021-01-01
期刊：
影响因子：
8.8
通讯作者：
Barata, Carlos
中科院分区：
文献类型：
--
作者：
Fuertes, Inmaculada;Barata, Carlos
神经递质是一种内源性代谢物，在生物体的化学突触中起着至关重要的作用。人们对他们了解污染物的神经毒性效应的分析测定越来越感兴趣。大型水蚤在多种神经递质和相关基因途径上与脊椎动物相似，在生态毒理学研究中有着广泛的应用，是进行这些环境研究的良好水生模型。在这项研究中，建立了一种准确而灵敏的分析17种神经递质及其相关前体和代谢物的方法。对方法的灵敏度、重现性、精密度和准确度进行了验证，并对基质效应进行了评价。作为方法有效性和适用性的独立探索者，该方法被应用于两种不同的场景。首先，用它研究了突变色氨酸水解酶D.大克隆的神经递质水平，证实了5-羟色胺及其代谢产物5-HIAA的缺失。此外，该方法还用于确定已知的影响不同神经递质系统的化合物的影响，并改变水蚤的行为。在所有治疗中，观察到13种分析的神经递质发生了显著变化，这些神经递质与被描述为受到这些神经化学物质影响的神经递质系统有关。这两项研究提供了影响大型金线虫神经递质系统的方式的结果，证实了所提出的方法的适用性，由于该生物适合于环境神经毒性研究，因此具有重要意义。(C)2020爱思唯尔有限公司。保留所有权利。
Neurotransmitters are endogenous metabolites that play a crucial role within an organism, at the chemical synapses. There is a growing interest in their analytical determination for understanding the neurotoxic effect of contaminants. Daphnia magna represents an excellent aquatic model for these environmental studies, due to its similarities with vertebrates in several neurotransmitters and related gene pathways and because of its wide application in ecotoxicological studies. Within this study, an accurate and sensible method of analysis of 17 neurotransmitters and related precursors and metabolites was developed. The method was validated in terms of sensitivity, reproducibility, precision, and accuracy, and also matrix effect was evaluated. As an independent probe of method validation and applicability, the method was applied to two different scenarios. First, it was used for the study of neurotransmitter levels in genetically mutated tryptophan hydrolase D. magna clones, confirming the absence of serotonin and its metabolite 5-HIAA. Additionally, the method was applied for determining the effects of chemical compounds known to affect different neurotransmitter systems and to alter Daphnia behavior. Significant changes were observed in 13 of the analyzed neurotransmitters across treatments, which were related to the neurotransmitter systems described as being affected by these neurochemicals. These two studies, which provide results on the ways in which the neurotransmitter systems in D. magna are affected, have corroborated the applicability of the presented method, of great importance due to the suitability of this organism for environmental neurotoxicity studies. (C) 2020 Elsevier Ltd. All rights reserved.