Proline and betaine provide protection to antioxidant and methylglyoxal detoxification systems during cold stress in Camellia sinensis (L.) O. Kuntze
Proline and betaine provide protection to antioxidant and methylglyoxal detoxification systems during cold stress in Camellia sinensis (L.) O. Kuntze
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DOI：
10.1007/s11738-008-0227-6
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发表时间：
2009-03-01
影响因子：
2.6
通讯作者：
Yadav, Sudesh Kumar
中科院分区：
文献类型：
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作者：
Kumar, Vinay;Yadav, Sudesh Kumar
本研究旨在监测低温胁迫下脯氨酸和甜菜碱对山茶抗氧化和甲基乙二醛解毒系统的影响。o . Kuntze。冷胁迫提高了茶芽（最年轻最顶端叶）MG和脂质过氧化水平。茶芽暴露于脯氨酸和甜菜碱后，这种增加受到抑制。茶芽暴露于脯氨酸和甜菜碱也有助于维持冷胁迫下的硫醇/二硫比。脯氨酸暴露增强了谷胱甘肽- s转移酶和谷胱甘肽还原酶（GR）活性，而甜菜碱暴露只增加了GR活性。此外，还研究了脯氨酸/甜菜碱对参与MG解毒的乙二醛酶途径酶的影响，该途径酶包括乙二醛酶I和乙二醛酶II。脯氨酸和甜菜碱均表现出对茶芽冷胁迫下乙二醛酶I的保护作用和对乙二醛酶II的激活作用。因此，本研究提示脯氨酸和甜菜碱可能通过调节茶中MG和脂质过氧化的形成以及激活或保护一些抗氧化酶和乙二醛酶途径的酶来保护茶中的冷胁迫。
The aim of this study was to monitor the influence of proline and betaine exposure on antioxidant and methylglyoxal (MG) detoxification system during cold stress in Camellia sinensis (L.) O. Kuntze. Cold stress enhanced MG and lipid peroxidation levels in tea bud (youngest topmost leaf). This increase was resisted upon the exposure of tea bud to proline and betaine. Exposure of tea bud with proline and betaine also help in maintaining thiol/disulfide ratio during cold stress. Proline exposure enhanced glutathione-S-transferase and glutathione reductase (GR) activity, while betaine exposure increased only GR activity during cold stress. Furthermore, effect of proline/betaine was studied on glyoxalase pathway enzymes that are involved in MG detoxification and comprise of two enzymes glyoxalase I and glyoxalase II. Both proline and betaine showed protective effect on glyoxalase I and activating effect on glyoxalase II during cold stress in tea bud. This investigation, therefore, suggest that proline and betaine might provide protection to cold stress in tea by regulating MG and lipid peroxidation formation as well as by activating or protecting some of antioxidant and glyoxalase pathway enzymes.