Platycodin D and 2"-o-acetyl-polygalacin D2 isolated from Platycodon grandiflorum protect ischemia/reperfusion injury in the gerbil hippocampus
Platycodin D and 2"-o-acetyl-polygalacin D2 isolated from Platycodon grandiflorum protect ischemia/reperfusion injury in the gerbil hippocampus
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DOI：
10.1016/j.brainres.2009.05.005
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发表时间：
2009-07-07
期刊：
影响因子：
2.9
通讯作者：
Kang, Il-Jun
中科院分区：
文献类型：
--
作者：
Choi, Jung Hoon;Yoo, Ki-Yeon;Kang, Il-Jun
Platycodi radix被用作几种情况的民间补救措施。在这项研究中，我们研究了五种主要提取物：deapioplatycoside E（DPE），platycoside E（PE），platyconic acid A（PA），platycodin D（PD）和2”-o-acetyl-polygalacin D2（PD 2）在缺血/再灌注（UR）后4天或10天从P. radix分离的海马CA 1区（CA 1）的神经保护作用。在缺血手术前10天，通过腹腔注射（5 mg/kg/天）将每种浸提液给予沙土鼠，并在I/R后4或10天处死沙土鼠。I/R后，溶剂处理组中CA 1区域的皮层中Fluorojade B（F-J B，神经变性的标志物）阳性（（+））神经元显著增加。在DPE、PE和PA处理组中观察到类似的模式;然而，在PD和PD 2处理组中，F-J B+神经元数量较少。我们还观察到，激活的星形胶质细胞和小胶质细胞在CA 1区I/R后被PD和PD 2治疗。此外，我们发现PD和PD 2治疗组锥体层Cu，Zn-超氧化物歧化酶（SOD 1）免疫反应性与假手术组相似，PD和PD 2治疗组I/R后考克斯-2（+）和NF-κ B B+细胞显著低于溶剂治疗组。这些结果表明，PD和PD 2从缺血性损伤中拯救CA 1区的神经元。(C)2009爱思唯尔有限公司版权所有。
Platycodi radix is used as a folk remedy for several conditions. In this study, we investigated the neuroprotective effects of five major extracts; deapioplatycoside E (DPE), platycoside E (PE), platyconic acid A (PA), platycodin D (PD) and 2"-o-acetyl-polygalacin D2 (PD2) isolated from the P. radix in the hippocampal CA1 region (CA1) 4 or 10 days after ischemia/reperfusion (UR). Each extract was administered into gerbils with intraperitoneal injection (5 mg/kg/day) 10 days before ischemic surgery and the gerbils were sacrificed 4 or 10 days after I/R. Fluorojade B (F-J B, a marker for neurodegeneration) positive ((+)) neurons increased significantly in the stratum pyramidale of the CA1 region in the vehicle-treated group after I/R. A similar pattern was observed in the DPE-, PE- and PA-treated groups; however, in the PD- and PD2-treated groups, F-J B+ neurons were small in number. We also observed that activations of astrocytes and microglia in the CA1 region after I/R were blocked by the PD- and PD2 treatments. In addition, we found that Cu,Zn-superoxide dismutase (SOD1) immuno reactivity in the pyramidal layer of the PD- and PD2-treated groups was similar to that of the sham group and COX-2(+) and NF-kappa B+ cells were significantly lower in the PD- and PD2-treated group than those in the vehicle-treated group after I/R. These results suggest that PD and PD2 rescue neurons in the CA1 region from an ischemic damage. (C) 2009 Elsevier B.V. All rights reserved.