Genetic elimination of rod/cone coupling reveals the contribution of the secondary rod pathway to the retinal output.
Genetic elimination of rod/cone coupling reveals the contribution of the secondary rod pathway to the retinal output.
复制标题
视杆细胞/视锥细胞耦合的遗传消除揭示了次级视杆细胞通路对视网膜输出的贡献。
DOI：
10.1126/sciadv.abm4491
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发表时间：
2022
期刊：
影响因子：
13.6
通讯作者：
Ribelayga,ChristopheP
中科院分区：
文献类型：
--
作者：
Jin,Nange;Tian,Lian-Ming;Fahrenfort,Iris;Zhang,Zhijing;Postma,Friso;Paul,DavidL;Massey,StephenC;Ribelayga,ChristopheP
在视网膜中，来自视杆和视锥光感受器的信号可以通过不同的途径到达视网膜神经节细胞（RGCs）-输出神经元。然而，很少有人知道这些途径的确切灵敏度和工作范围。以前，我们创建了视杆细胞或视锥细胞特异性Cx 36敲除（KO）小鼠品系。这两条线都缺乏视杆/视锥电耦合，因此提供了一种选择性地去除第二视杆通路的方法。我们测量了野生型小鼠RGCs中初级视杆细胞通路的阈值。在药物阻断初级视杆细胞通路的情况下，阈值升高。在Cx 36 KO中去除该次级成分以暴露第三视杆细胞通路的阈值，其仍然低于视锥细胞阈值。反过来，锥阈值估计通过几个独立的方法。我们的工作定义了二级视杆细胞通路的功能，并描述了不同通路对视网膜输出的附加贡献。
In the retina, signals originating from rod and cone photoreceptors can reach retinal ganglion cells (RGCs)—the output neurons—through different pathways. However, little is known about the exact sensitivities and operating ranges of these pathways. Previously, we created rod- or cone-specificCx36knockout (KO) mouse lines. Both lines are deficient in rod/cone electrical coupling and therefore provide a way to selectively remove the secondary rod pathway. We measured the threshold of the primary rod pathway in RGCs of wild-type mice. Under pharmacological blockade of the primary rod pathway, the threshold was elevated. This secondary component was removed in theCx36KOs to unmask the threshold of the third rod pathway, still below cone threshold. In turn, the cone threshold was estimated by several independent methods. Our work defines the functionality of the secondary rod pathway and describes an additive contribution of the different pathways to the retinal output.