Distribution of ion channels in ascidian eggs and zygotes.
Distribution of ion channels in ascidian eggs and zygotes.
复制标题
海鞘卵和受精卵中离子通道的分布。
DOI：
10.1016/0014-4827(89)90197-3
复制
发表时间：
1989
影响因子：
3.7
通讯作者：
Talevi,R
中科院分区：
文献类型：
--
作者：
Dale,B;Talevi,R
Ascidian eggs and zygotes were whole-cell voltage-clamped and inward membrane currents, generated by stepping the membrane potential, studied from fertilization up to cytokinesis. Currents, induced by changing the voltage in steps from −80 to −30 mV, or to 0 mV, had maximum amplitudes which ranged from 400 to 1200 pA in the unfertilized egg and 100 to 1300 pA in the zygote. At 5 to 10 min after fertilization it was not possible to generate inward currents owing to the activity of nonspecific fertilization channels. Preceding cytokinesis, we observed a reduction in amplitude of the inward currents. By cutting eggs and zygotes into fragments, we have shown that the ion channels generating these inward currents are symmetrically distributed over the egg plasma membrane, but regionalized in the zygote with a maximum density at the animal pole.
