Inverted repetitions in the chromosome of herpes simplex virus.
Inverted repetitions in the chromosome of herpes simplex virus.
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单纯疱疹病毒染色体的反向重复。
DOI：
10.1101/sqb.1974.039.01.080
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
N. Berthelot
中科院分区：
文献类型：
--
作者：
P. Sheldrick;N. Berthelot
单链DNA有时会自我折叠，通过碱基配对形成双链区域。这种行为是诊断重复，互补的核苷酸序列在链，这是相对于另一个倒置。这种反向重复的例子(ABC…通过电子显微镜在细菌质粒（Sharp et al. 1973）、噬菌体Mu （Hsu and Davidson 1974）、真核染色质（Thomas et al. 1974）和两种动物病毒（腺病毒（Garon et al. 1972; Wolfson and Dressler 1972）和腺相关病毒（Koczot et al. 1973; Gerry et al. 1973）的DNA中发现了C' b ' a '）。普通的重复(ABC…ABC)也存在于多种噬菌体（参见Thomas and MacHattie 1967）、真核染色质（Thomas et al. 1974）和腺相关病毒（Gerry et al. 1973）的DNA中。当不作为内聚末端暴露时，这些序列可以通过外切酶III消化后在双分子中形成环状来检测（MacHattie et al. 1967）。在这篇通讯中，我们描述了电子显微镜研究，显示完整的单链单纯疱疹病毒（HSV） DNA以不同的方式折叠自己，产生几种结构形式。其中一种形式，两个由双链区域连接的圆圈，需要在单链中存在两个反向重复。此外，我们观察到用核酸外切酶ii处理天然HSV DNA分子后形成双环，表明在链末端存在普通重复。
Single DNA strands will sometimes fold back on themselves and form duplex regions by base pairing. This behavior is diagnostic of repeated, complementary nucleotide sequences in the strand, which are inverted with respect to one another. Examples of such inverted repetitions (ABC... C'B'A') have been found by electron microscopy in DNA from bacterial plasmids (Sharp et al. 1973), bacteriophage Mu (Hsu and Davidson 1974), eukaryotic chromatin (Thomas et al. 1974) and two animal viruses, adenovirus (Garon et al. 1972; Wolfson and Dressler 1972) and adeno-associated virus (Koczot et al. 1973; Gerry et al. 1973). Ordinary repetitions (ABC... ABC) are also found in DNA from a variety of bacteriophages (for review, see Thomas and MacHattie 1967), eukaryotic chromatin (Thomas et al. 1974), and adeno-associated virus (Gerry et al. 1973). When not exposed as cohesive ends, these sequences can be detected by circle formation in duplex molecules after digestion with exonuclease III (MacHattie et al. 1967).In this communication we describe electron microscope studies showing that intact single strands of herpes simplex virus (HSV) DNA fold back on themselves in different ways to generate several structural forms. One of these forms, two circles joined by a duplex region, requires the presence of two inverted repetitions in the single strand. In addition, we observed the formation of duplex circles after treatment of native HSV DNA molecules with exonuclease Ill, indicating the presence of an ordinary repetition at the strand termini.