UV-induced differential gene expression in rice cultivars analyzed by SSH
UV-induced differential gene expression in rice cultivars analyzed by SSH
复制标题
通过 SSH 分析紫外线诱导的水稻品种差异基因表达
DOI：
10.1007/s10725-009-9411-y
复制
发表时间：
2009-12-01
影响因子：
4.2
通讯作者：
Lin, Wen-Xiong
中科院分区：
文献类型：
--
作者：
Fang, Chang-Xun;Wu, Xing-Chun;Lin, Wen-Xiong
研究了两种水稻基因型--抗UV-B（UV-B）品种Lemont和抗UV-B品种Dular--对UV-B辐射响应的基因表达差异。采用抑制性消减杂交和实时定量逆转录聚合酶链反应进行分析。结果表明，增强的UV-B辐射可诱导防御反应相关基因的差异表达。HECT结构域蛋白、类囊体抗坏血酸过氧化物酶、类受体蛋白激酶、Rad 6（泛素载体蛋白）、苯丙氨酸解氨酶、4-香豆酸：CoA连接酶和查尔酮合成酶等基因在两个水稻品种中的表达均不同程度地上调：Lemont品种的基因表达量高于Dular品种。在通用应激蛋白（USP）的表达模式中发现了很大的差异：在Dular中显示下调，但在Lemont中显示上调。因此，暴露于UV-B辐射对水稻的基因表达有显着影响。为了响应紫外线照射，不同的抗紫外线B水稻品种最初会诱导分子反应。这种反应可能激活次级代谢途径，进而增加紫外线保护物质（类黄酮）的合成，以防止UV-B损伤，并诱导相关mRNA的转录，以修复受损的DNA。
The differences in gene expression between two rice genotypes-the ultraviolet-B (UV-B)-resistant cultivar Lemont and the UV-B-sensitive cultivar Dular-in response to irradiation with UV-B were studied. Suppression subtractive hybridization and real-time quantitative reverse transcriptase polymerase chain reaction were used in the analysis. The results showed that enhanced UV-B radiation could induce differential expression of the genes related to defense response. The expression of genes encoding HECT domain-containing protein, thylakoid-bound ascorbate peroxidase, receptor-like protein kinase, Rad6 (ubiquitin carrier protein), phenylalanine ammonia-lyase, 4-coumarate: CoA ligase and chalcone synthase was up-regulated to different extents in the two rice cultivars: The cultivar Lemont exhibited a higher increase in the gene expression compared with the cultivar Dular. A large difference was found in the expression pattern of the universal stress protein (USP): showing down-regulation in Dular but up-regulation in Lemont. Hence exposure to UV-B radiation has a significant effect on gene expression in rice. In response to UV exposure, a molecular response is initially induced in different UV-B-resistant rice cultivars. This response may activate secondary metabolic pathways, in turn increase the synthesis of UV-protective substances (flavonoids) to prevent UV-B damage and induce the transcription of the relevant mRNAs for repairing the damaged DNA.