Observation and characterization of microvascular vasomotion using erythrocyte mediated ICG angiography (EM-ICG-A)
Observation and characterization of microvascular vasomotion using erythrocyte mediated ICG angiography (EM-ICG-A)
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DOI：
10.1016/j.mvr.2017.02.006
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发表时间：
2017-09-01
影响因子：
3.1
通讯作者：
Kling, Ronn
中科院分区：
文献类型：
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作者：
Flower, Robert W.;Kling, Ronn
基于观察毛细血管中含有ICG染料的自体再注射红细胞的动力学，证明了一种表征微血管血管舒张状态的临床方法。由于血管舒缩的一种表现是短暂的红细胞暂停，在一个固定的观察期间，血管舒缩状态的特征是暂停红细胞的数量作为暂停时间的函数的直方图，然后计算长暂停红细胞与短暂停红细胞的比例。该方法首先应用于麻醉猴眼的后极视网膜血管，并将正常呼吸空气时的血管舒缩状态与o2呼吸诱导的血管舒缩状态进行比较，o2呼吸在成熟眼中引起轻度小动脉血管收缩。随后，其他拮抗剂对正常血管小动脉舒张状态（长期实验诱导的高眼压和支静脉闭塞，以及组织水肿）的影响也具有类似的特征，并将结果与基线呼吸时的结果进行了比较。初步探讨了将血管舒缩状态直方图表征应用于人眼和皮肤的可行性。(C) 2017爱思唯尔公司版权所有。
A clinical method for characterizing the state of micro-vasculature vasomotion is demonstrated, based on observing in capillaries the dynamics of autologously re-injected erythrocytes containing ICG dye. Since a manifestation of vasomotion is transient erythrocyte pausing, vasomotion state within a field of capillaries is characterized by an histogram plot of the number of paused erythrocytes as a function of pause duration during a fixed period of observation, then the ratio of long-pausing to short-pausing erythrocytes was calculated. The method was first applied to the posterior pole retinal vasculatures of anesthetized-monkey eyes, and normal vasomotion state during air-breathing was compared to the state induced by O-2-breathing, known to cause mild arteriolar vasoconstriction in the mature eye. Subsequently, the effects of other antagonists to normal arteriolarvasotonia state (long-standing experimentally-induced ocular hypertension and branch-vein occlusion, as well as tissue edema) were similarly characterized and the results compared to those obtained during baseline air-breathing. The feasibility of applying the histogram characterization of vasomotion state to human eyes and skin was also preliminarily explored. (C) 2017 Elsevier Inc. All rights reserved.