Uncovering Hierarchical Regulation among MYB-bHLH-WD40 Proteins and Manipulating Anthocyanin Pigmentation in Rice.
Uncovering Hierarchical Regulation among MYB-bHLH-WD40 Proteins and Manipulating Anthocyanin Pigmentation in Rice.
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DOI：
10.3390/ijms23158203
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发表时间：
2022-07-26
影响因子：
5.6
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中科院分区：
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花色素苷在水稻的各个器官中积累，并且已经阐明了参与果皮、船体、叶、尖和柱头等特定器官色素形成的调控基因。然而，水稻秆色素沉着的相应基因尚未阐明。MYB-bHLH-WD 40（MBW）复合物在植物花青素合成途径中起着重要的调控作用。然而，MBW的核心成员和这些成员之间的等级调节在水稻中没有完全阐明。在这里，通过图位克隆，我们确定了秆特异性色素沉着基因S1的等位基因也是已知的船体/果皮色素沉着。我们还阐明了WD 40蛋白编码基因WA 1是水稻花色素苷合成所必需的。在MBW成员间的级联调控中，S1（bHLH）作为主基因激活C1（MYB）的表达，C1再激活植物特有的WA 1（WD 40）的表达。这使得MBW成员能够以共同的方式协调以有效地调节花青素苷生物合成基因。在此基础上，我们探索了水稻花色素苷合成所需的最小基因集。这些发现将帮助我们设计新的水稻品种，根据需要在特定器官中积累花青素。
Anthocyanins accumulate in various organs of rice, and the regulatory genes involved in pigmentation of specific organs, such as pericarp, hull, leaf, apiculus, and stigma have been elucidated. However, the corresponding gene for rice culm pigmentation has not been clarified. The well-known MYB-bHLH-WD40 (MBW) complex plays vital role in regulating the anthocyanin biosynthesis pathway in plants. However, the core members of MBW and the hierarchical regulation between these members are not fully elucidated in rice. Here, by map-based cloning, we identified the culm-specific pigmentation gene S1 whose alleles are also known for hull/pericarp pigmentation. We also clarified that one WD40 protein encoding gene, WA1, is indispensable for anthocyanin biosynthesis in rice. In the cascading regulation among MBW members, S1 (bHLH) acts as the master gene by activating the expression of C1 (MYB), and then C1 activates the expression of WA1 (WD40), which is unique in plant species. This enables MBW members to be coordinated in a common way to efficiently regulate anthocyanin biosynthesis genes. Based on these studies, we explored the minimal gene set required for anthocyanin biosynthesis in rice. These findings will help us design new rice varieties with anthocyanin accumulation in specific organs as needed.