An alternative workflow for molecular detection of SARS-CoV-2-escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020
An alternative workflow for molecular detection of SARS-CoV-2-escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020
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DOI：
10.2807/1560-7917.es.2020.25.14.2000398
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发表时间：
2020-04-09
期刊：
影响因子：
19
通讯作者：
Rosenstierne, Maiken Worsoe
中科院分区：
文献类型：
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作者：
Fomsgaard, Anna S.;Rosenstierne, Maiken Worsoe
世界卫生组织宣布由新发现的SARS-CoV-2引起的COVID-19为大流行病。由于对提取SARS-CoV-2 RNA用于后续RT-qPCR诊断的试剂和/或试剂盒的需求不断增长，全球范围内存在短缺的风险。检测灵敏度为97.4%（95%CI：86.2-99.9%），我们描述了一种简单，快速，替代的工作流程，用于SARS-CoV-2的分子检测，其中样品在常用的RT-qPCR程序之前在98 ℃下简单地热处理5分钟。
The World Health Organization has declared COVID-19 caused by the newly discovered SARS-CoV-2 a pandemic. Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI: 86.2-99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 min at 98 degrees C before a commonly-used RT-qPCR procedure.