Application of agarose microcapsules to allo-islet transplantation in a canine model.
Application of agarose microcapsules to allo-islet transplantation in a canine model.
复制标题
琼脂糖微胶囊在犬同种异体胰岛移植中的应用。
DOI：
10.1016/s0041-1345(97)01374-2
复制
发表时间：
1998
影响因子：
0.9
通讯作者：
T. Tsuji
中科院分区：
文献类型：
--
作者：
H. Tashiro;H. Iwata;G. Warnock;Y. Ikada;T. Tsuji
材料与方法以无免疫抑制的5只全胰腺切除的糖尿病犬为受体，测定微囊化胰岛的功能。每隔3-7天，通过导管将一定数量的来自近交系供体的微囊化胰岛移植到胰腺切除受者的大网膜组织中，直到达到总剂量的胰岛，在不注射外源性胰岛素的情况下恢复空腹血糖正常。每天监测空腹血糖，用化学试剂盒检测，每7天用Beckman 2型血糖分析仪精确测定一次。分别于移植前和移植后1个月进行葡萄糖耐量试验(0.5g葡萄糖/kg体重)。根据注射葡萄糖后5、10、30和60分钟的血糖水平计算K值(血糖水平下降，%/分钟)。
MATERIALS AND METHODSFunctional efficacy of the microencapsulated islets was determined using five totally pancreatectomized diabetic dogs as recipients without immunosuppression. Defined quantity of microencapsulated islets from outbred mongrel donors was grafted through the catheter into omental tissue of the pancreatectomized recipient at 3-to 7-day intervals until a total dose of islets was achieved that restored fasting normoglycemia without exogenous insulin injection. Fasting blood glucose was monitored daily, using chemstrip tapes and precisely determined by a Beckman glucose analyzer type 2 every 7 days. Intravenous glucose tolerance tests (0.5 g glucose/kg body weight) were performed preoperatively and at 1-month postimplantation. K values (decline of glucose level,%/min) were calculated from plasma glucose levels at 5, 10, 30, and 60 minutes after injection of glucose.