Radioimmunoassay for amatoxins by use of a rapid, 125I-tracer-based system.
Radioimmunoassay for amatoxins by use of a rapid, 125I-tracer-based system.
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使用基于 125I 示踪剂的快速系统对鹅膏毒素进行放射免疫测定。
DOI：
10.1093/clinchem/32.9.1751
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发表时间：
1986
影响因子：
9.3
通讯作者：
D. Vonderschmitt
中科院分区：
文献类型：
--
作者：
R. Andres;W. Frei;K. Gautschi;D. Vonderschmitt
在这个新的放射免疫测定系统测定鹅膏蕈毒素在尿液和血浆中，一种新的化学方法用于抗原和125 I-示踪剂的生产，基于解毒的α-鹅膏蕈碱衍生物（aldoamanitin）。总分析时间（包括数据处理）小于100 min。尿液的最低可检测浓度为1 μ g/L，血浆为0.1 μ g/L。在具有临床意义的范围内，运行内CV小于8%。这种新的基于125 I的检测方法在速度、精度和无干扰方面比现有的3 H技术有了显著的改进。
In this new radioimmunoassay system for determination of amatoxins in urine and plasma, a novel chemical approach is used for antigen and 125I-tracer production, based on a detoxified alpha-amanitin derivative (aldoamanitin). Total assay time, including data processing, is less than 100 min. The lowest detectable concentration is 1 microgram/L for urine, 0.1 microgram/L for plasma. In the clinically significant range, within-run CVs are less than 8%. This new 125I-based assay is a significant improvement over existing 3H technology in terms of speed, precision, and freedom from interference.