Establishment of a HEK293 cell line by CRISPR/Cas9-mediated luciferase knock-in to study transcriptional regulation of the human SREBP1 gene
Establishment of a HEK293 cell line by CRISPR/Cas9-mediated luciferase knock-in to study transcriptional regulation of the human SREBP1 gene
复制标题
通过CRISPR/Cas9介导的荧光素酶敲入建立HEK293细胞系以研究人类SREBP1基因的转录调控
DOI：
10.1007/s10529-018-2608-2
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发表时间：
2018-12-01
影响因子：
2.7
通讯作者：
Xia, Haibin
中科院分区：
文献类型：
--
作者：
Li, Zihang;Zhao, Junli;Xia, Haibin
目的建立内源性SREBP 1启动子调控的荧光素酶报告基因敲入HEK 293细胞系，研究SREBP 1基因的转录调控。在HEK 293-SREBP 1-T2 A-luciferase-KI细胞系cell line.ConclusionsWe成功地产生了一种新的荧光素酶敲入报告系统，这将是非常有用的研究SREBP 1基因的转录调控和筛选药物或化学分子，调节SREBP 1基因的表达。
ObjectivesTo establish a HEK293 cell line with a luciferase knock-in reporter controlled by the endogenous SREBP1 promoter for investigating transcriptional regulation of the SREBP1 gene.ResultsPCR confirmed the site-specific integration of a single copy of the exogenous luciferase gene into one allele of the genome and a 14 bp deletion of the targeted sequence in the other. Luciferase activity was directly correlated with the promoter activity of the endogenous SREBP1 gene in the HEK293-SREBP1-T2A-luciferase-KI cell line cell line.ConclusionsWe successfully generated a novel luciferase knock-in reporter system, which will be very useful for studying transcriptional regulation of the SREBP1 gene and for screening drugs or chemical molecules that regulate SREBP1 gene expression.