Anti-metastasis Activity of Black Rice Anthocyanins Against Breast Cancer: Analyses Using an ErbB2 Positive Breast Cancer Cell Line and Tumoral Xenograft Model
Anti-metastasis Activity of Black Rice Anthocyanins Against Breast Cancer: Analyses Using an ErbB2 Positive Breast Cancer Cell Line and Tumoral Xenograft Model
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黑米花青素对乳腺癌的抗转移活性：使用 ErbB2 阳性乳腺癌细胞系和肿瘤异种移植模型进行分析
DOI：
10.7314/apjcp.2014.15.15.6219
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发表时间：
2014-01-01
影响因子：
--
通讯作者：
Li, Sui-Yan
中科院分区：
文献类型：
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作者：
Luo, Li-Ping;Han, Bin;Li, Sui-Yan
背景：来自动物、流行病学和临床研究的越来越多的证据表明，膳食中的花青素具有预防包括癌症在内的慢性疾病的潜力。还值得注意的是，人表皮生长因子受体2（ErbB2）蛋白的过度表达或ErbB2基因扩增已被列为乳腺癌转移和复发风险更高的指标。
材料与方法：本实验在体内和体外研究了黑米花青素（BRACs）对ErbB2阳性乳腺癌细胞的抗转移作用。
结果：在携带ErbB2阳性乳腺癌细胞MDA - MB - 453异种移植物的BALB/c裸鼠中，口服给予BRACs（150mg/kg/天）可减少移植瘤的生长，抑制肺转移，并减少肺肿瘤结节。BRACs还以浓度依赖的方式抑制MDA - MB - 453细胞的迁移、黏附、运动和侵袭能力，同时伴有转移促进因子尿激酶型纤溶酶原激活剂（u - PA）活性的降低。
结论：总之，我们的结果表明，BRACs在体内和体外通过抑制转移促进分子，对ErbB2阳性的人乳腺癌细胞具有抗转移潜力。
Background: Increasing evidence from animal, epidemiological and clinical investigations suggest that dietary anthocyanins have potential to prevent chronic diseases, including cancers. It is also noteworthy that human epidermal growth factor receptor 2 (ErbB2) protein overexpression or ErbB2 gene amplification has been included as an indicator for metastasis and higher risk of recurrence for breast cancer. Materials and Methods: The present experiments investigated the anti-metastasis effects of black rice anthocyanins (BRACs) on ErbB2 positive breast cancer cells in vivo and in vitro. Results: Oral administration of BRACs (150 mg/kg/day) reduced transplanted tumor growth, inhibited pulmonary metastasis, and decreased lung tumor nodules in BALB/c nude mice bearing ErbB2 positive breast cancer cell MDA-MB-453 xenografts. The capacity for migration, adhesion, motility and invasion was also inhibited by BRACs in MDA-MB-453 cells in a concentration dependent manner, accompanied by decreased activity of a transfer promoting factor, urokinase-type plasminogen activator (u-PA). Conclusions: Together, our results indicated that BRACs possess anti-metastasis potential against ErbB2 positive human breast cancer cells in vivo and in vitro through inhibition of metastasis promoting molecules.