Isolation and Characterization of an Enterobacter cloacae Strain That Reduces Hexavalent Chromium under Anaerobic Conditions
Isolation and Characterization of an Enterobacter cloacae Strain That Reduces Hexavalent Chromium under Anaerobic Conditions
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DOI：
10.1128/aem.55.7.1665-1669.1989
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发表时间：
1989-07
影响因子：
4.4
通讯作者：
Pinfei Wang;Tsukasa Mori;K. Komori;M. Sasatsu;K. Toda;H. Ohtake
中科院分区：
文献类型：
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作者：
Pinfei Wang;Tsukasa Mori;K. Komori;M. Sasatsu;K. Toda;H. Ohtake
从活性污泥中分离到一株能还原六价铬(铬酸盐)的阴沟肠杆菌(HO1)。该菌在好氧和厌氧条件下均对铬酸盐产生抗性。只有阴沟肠杆菌的厌氧培养显示出铬酸盐还原。在厌氧培养中，随着还原过程的进行，黄色变为白色，浊度增加，表明生成了不溶的氢氧化铬。阴沟肠杆菌可能以厌氧方式利用有毒铬酸盐作为电子受体，因为(I)阴沟肠杆菌HO1的厌氧生长伴随着培养液中有毒铬酸盐的减少，(Ii)氧迅速抑制铬酸盐还原活性，以及(Iii)甘油或醋酸盐培养细胞的还原速度快于葡萄糖培养细胞。阴沟肠杆菌HO1在pH 6.0~8.5(最适pH 7.0)和10~40℃(最适30℃)下可观察到铬酸盐还原反应。
An Enterobacter cloacae strain (HO1) capable of reducing hexavalent chromium (chromate) was isolated from activated sludge. This bacterium was resistant to chromate under both aerobic and anaerobic conditions. Only the anaerobic culture of the E. cloacae isolate showed chromate reduction. In the anaerobic culture, yellow turned white with chromate and the turbidity increased as the reduction proceeded, suggesting that insoluble chromium hydroxide was formed. E. cloacae is likely to utilize toxic chromate as an electron acceptor anaerobically because (i) the anaerobic growth of E. cloacae HO1 accompanied the decrease of toxic chromate in culture medium, (ii) the chromate-reducing activity was rapidly inhibited by oxygen, and (iii) the reduction occurred more rapidly in glycerol- or acetate-grown cells than in glucose-grown cells. The chromate reduction in E. cloacae HO1 was observed at pH 6.0 to 8.5 (optimum pH, 7.0) and at 10 to 40°C (optimum, 30°C).