Vaccination of mice with baculovirus-infected insect cells expressing antigenic proteins.
Vaccination of mice with baculovirus-infected insect cells expressing antigenic proteins.
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DOI：
10.1002/0471142735.im0215s85
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发表时间：
2009-04
影响因子：
--
通讯作者：
Slansky, Jill E
中科院分区：
文献类型：
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作者：
Jordan, Kimberly R;Crawford, Fran;Kappler, John W;Slansky, Jill E
Methods to induce antigen-specific immune responses in mice using insect cells infected with recombinant baculoviruses are described in this unit. Although this vaccine strategy has been used to generate both antibody and T cell responses, it has been more thoroughly characterized for the peptide-specific cytotoxic T cell responses. Nonspecific responses to the vaccine vehicle are controlled for by vaccinating with insect cells infected with baculoviruses encoding irrelevant antigens or no antigen. The baculovirus-infected insect cells alone are an effective immune adjuvant to elicit antigen-specific T cells. Overall, immune responses generated using this approach are similar to those generated by more conventional vaccine strategies.
