NTPDase2 and the P2Y1 receptor are not required for mammalian eye formation.
NTPDase2 and the P2Y1 receptor are not required for mammalian eye formation.
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NTPDase2 和 P2Y1 受体不是哺乳动物眼睛形成所必需的。
DOI：
10.1007/s11302-014-9440-5
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发表时间：
2015
影响因子：
3.5
通讯作者：
Zimmermann,Herbert
中科院分区：
文献类型：
--
作者：
Gampe,Kristine;Haverkamp,Silke;Robson,SimonC;Gachet,Christian;Hüser,Laura;Acker-Palmer,Amparo;Zimmermann,Herbert
脊椎动物眼睛的形成是由分子网络的保守模式控制的。同源框转录因子在视网膜的建立和维护中发挥着至关重要的作用。 Massé 等人之前的一项研究。 (Nature, 449: 1058–62, 2007) 使用吗啉代敲低技术鉴定出外切核苷酸酶 NTPDase2 和 P2Y1 受体是爪蛙胚胎眼睛形成的重要元件。为了研究类似的重要机制是否会在哺乳动物眼睛发育中发挥作用，我们分析了小鼠 Entpd2 或 P2ry1 的 KO 以及 Entpd2/P2ry1 的双重 KO。这些小鼠发育出正常的眼睛。为了识别分子身份或视网膜细胞元件排列的潜在缺陷，我们使用各种视网膜标记物进行了免疫组织学分析。对单敲除和双敲除小鼠的分析表明，NTPDase2 和 P2Y1 受体不是小鼠眼睛形成所必需的，正如先前在非洲爪蟾眼睛发育中所显示的那样。
Eye formation in vertebrates is controlled by a conserved pattern of molecular networks. Homeobox transcription factors are crucially involved in the establishment and maintenance of the retina. A previous study of Massé et al. (Nature, 449: 1058–62, 2007) using morpholino knockdown identified the ectonucleotidase NTPDase2 and the P2Y1receptor as essential elements for eye formation in embryos of the clawed frogXenopus laevis. In order to investigate whether a similarly essential mechanism would be active in mammalian eye development, we analyzed mice KO forEntpd2orP2ry1as well as double KO forEntpd2/P2ry1. These mice developed normal eyes. In order to identify potential deficits in the molecular identity or in the arrangement of the cellular elements of the retina, we performed an immunohistological analysis using a variety of retinal markers. The analysis of single and double KO mice demonstrated that NTPDase2 and P2Y1receptors are not required for murine eye formation, as previously shown for eye development inXenopus laevis.