CRISPR/Cas mediated genome editing in potato: Past achievements and future directions
CRISPR/Cas mediated genome editing in potato: Past achievements and future directions
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DOI：
10.1016/j.plantsci.2022.111474
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发表时间：
2022-09-29
期刊：
影响因子：
5.2
通讯作者：
Qi, Yiping
中科院分区：
文献类型：
--
作者：
Tuncel, Aytug;Qi, Yiping
基因组工程正在重塑植物生物技术和农业。使用最近开发的基因编辑技术进行作物改良现在比以往任何时候都更容易，更快，更精确。尽管马铃薯被认为是全球粮食安全作物，但它并没有从这些技术的多样化收集中受益。栽培马铃薯独特的遗传特征，如四体遗传、高基因组杂合性和近交衰退，阻碍了这一重要作物的常规育种。因此，基因组编辑为马铃薯性状改良提供了一个极好的工具库。此外，使用特定的转化方案，可以工程化无转基因的商业品种。在这篇综述中，我们首先描述了过去在马铃薯基因组编辑方面取得的成就，并强调了这些努力中缺失的一些方面。然后，我们讨论了马铃薯基因组编辑的技术挑战，并提出了克服这些困难的方法。最后，我们讨论了尚未在马铃薯中探索的基因组编辑应用，并指出了文献中缺失的一些地方。
Genome engineering has been re-shaping plant biotechnology and agriculture. Crop improvement using the recently developed gene editing techniques is now easier, faster, and more precise than ever. Although considered to be a global food security crop, potato has not benefitted enough from diverse collection of these techniques. Unique genetic features of cultivated potatoes such as tetrasomic inheritance, high genomic heterozygosity, and inbreeding depression hamper conventional breeding of this important crop. Therefore, genome editing provides an excellent arsenal of tools for trait improvement in potato. Moreover, using specific transformation protocols, it is possible to engineer transgene free commercial varieties. In this review we first describe the past achievements in potato genome editing and highlight some of the missing aspects of these efforts. Then, we discuss about technical challenges of genome editing in potato and present approaches to overcome these difficulties. Finally, we talk about genome editing applications that have not been explored in potato and point out some of the missing venues in literature.