Synthetic and tissue-derived models for studying rigidity effects on invadopodia activity.
Synthetic and tissue-derived models for studying rigidity effects on invadopodia activity.
复制标题
用于研究刚性对侵袭伪足活动影响的合成和组织衍生模型。
DOI：
10.1007/978-1-62703-538-5_10
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Parekh,Aron
中科院分区：
文献类型：
--
作者：
Weaver,AlissaM;Page,JonathanM;Guelcher,ScottA;Parekh,Aron
癌细胞通过组织的细胞外基质（ECM）侵袭是肿瘤进展和转移的关键步骤。富含肌动蛋白的亚细胞突起被认为是通过定位蛋白酶来促进这一过程，蛋白酶降解ECM并允许癌细胞渗透。我们已经在体外证明，外基质活性受外基质硬度的调节，这表明体内基质重塑也可能受组织力学特性的调节。为了以可控的方式研究刚性对侵入性活性的影响，我们开发了将可降解荧光基质分子共轭到可调节的合成底物上的测定方法。此外，我们还利用离体组织来源的底物来证实我们的发现。在本章中，我们提出了详细的方案，描述了我们的合成底物，聚丙烯酰胺凝胶和聚氨酯弹性体的合成和制备，用于这些基质降解试验，以及利用我们的组织衍生底物所需的步骤。
Invasion by cancer cells through the extracellular matrix (ECM) of tissues is a critical step in cancer progression and metastasis. Actin-rich subcellular protrusions known as invadopodia are thought to facilitate this process by localizing proteinases which degrade the ECM and allow for cancer cell penetration. We have shown in vitro that invadopodia activity is regulated by the rigidity of the ECM, which suggests that matrix remodeling in vivo may also be regulated by the mechanical properties of tissues. In order to study rigidity effects on invadopodia activity in a controlled manner, we have developed assays in which we have conjugated degradable fluorescent matrix molecules to tunable synthetic substrates. In addition, we have also utilized ex vivo tissue-derived substrates to corroborate our findings. In this chapter, we present detailed protocols describing the synthesis and preparation of our synthetic substrates, polyacrylamide gels and polyurethane elastomers, for use in these matrix degradation assays as well as the steps required to utilize our tissue-derived substrates.
登录
查看更多内容
聚合物的通用荧光
影响因子：
0.8
作者：
V. F. Gachkovskiĭ
通讯作者：
V. F. Gachkovskiĭ
DOI：
10.1158/1055-9965.715.13.5
发表时间：
2004-05
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
--
作者：
P. Vacek;B. Geller
通讯作者：
P. Vacek;B. Geller
影响因子：
3.4
作者：
Parekh, Aron;Ruppender, Nazanin S.;Weaver, Alissa M.
通讯作者：
Weaver, Alissa M.
DOI：
10.1007/bf01540646
发表时间：
1994-01-01
期刊：
Journal of Tissue Culture Methods
影响因子：
--
作者：
Chen, Wen-Tien;Yeh, Yunyun;Nakahara, Hirokazu
通讯作者：
Nakahara, Hirokazu
黑色素瘤侵袭的调节机制
影响因子：
4.8
作者：
H. Nakahara;M. Nomizu;S. K. Akiyama;Yoshihiko Yamada;Y. Yeh;Wen‐Tien Chen
通讯作者：
Wen‐Tien Chen