COMPARISON OF THE NUCLEOTIDE-SEQUENCE OF SOYBEAN 18S RIBOSOMAL-RNA WITH THE SEQUENCES OF OTHER SMALL-SUBUNIT RIBOSOMAL-RNAS
COMPARISON OF THE NUCLEOTIDE-SEQUENCE OF SOYBEAN 18S RIBOSOMAL-RNA WITH THE SEQUENCES OF OTHER SMALL-SUBUNIT RIBOSOMAL-RNAS
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DOI：
10.1007/bf02102358
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发表时间：
1985-01-01
影响因子：
3.9
通讯作者：
MEAGHER, RB
中科院分区：
文献类型：
--
作者：
ECKENRODE, VK;ARNOLD, J;MEAGHER, RB
本文报道了大豆核糖体小亚基RNA的核编码序列。大豆18S rRNA序列的1807个核苷酸（nt）包含在一个基因家族中，每个单倍体基因组约有800个密切相关的成员。该序列与玉米（1805 nt）、酵母（1789 nt）、非洲爪蟾（1825 nt）、大鼠（1869 nt）和大肠杆菌（1541 nt）的核糖体小亚基RNA进行了比较。显着的序列同源性之间观察到的真核小亚基rRNA检查，和一些序列同源性之间观察到的真核和原核小亚基rRNA。保守区域被发现散布在高度分化的序列之间。这些比较的意义进行评估，使用计算机模拟的随机序列模型。大豆18S rRNA的二级结构的一个尝试性的模型，并在序列内的各种保守区域的功能的上下文中进行了讨论。在此模型的基础上，短碱基配对序列定义的四个结构和功能域的所有18S rRNA被认为是很保守的。本文还讨论了其它保守的大豆18SrRNA序列在蛋白质合成中的潜在作用。
We present the sequence of the nuclearencoded ribosomal small-subunit RNA from soybean. The soybean 18S rRNA sequence of 1807 nucleotides (nt) is contained in a gene family of approximately 800 closely related members per haploid genome. This sequence is compared with the ribosomal small-subunit RNAs of maize (1805 nt), yeast (1789 nt),Xenopus(1825 nt), rat (1869 nt), andEscherichia coli(1541 nt). Significant sequence homology is observed among the eukaryotic small-subunit rRNAs examined, and some sequence homology is observed between eukaryotic and prokaryotic small-subunit rRNAs. Conserved regions are found to be interspersed among highly diverged sequences. The significance of these comparisons is evaluated using computer simulation of a random sequence model. A tentative model of the secondary structure of soybean 18S rRNA is presented and discussed in the context of the functions of the various conserved regions within the sequence. On the basis of this model, the short basepaired sequences defining the four structural and functional domains of all 18S rRNAs are seen to be well conserved. The potential roles of other conserved soybean 18S rRNA sequences in protein synthesis are discussed.