Genome-wide transcriptome profiles reveal how Bacillus subtilis lipopeptides inhibit microsclerotia formation in Verticillium dahliae
Genome-wide transcriptome profiles reveal how Bacillus subtilis lipopeptides inhibit microsclerotia formation in Verticillium dahliae
复制标题
全基因组转录组图谱揭示了枯草芽孢杆菌脂肽如何抑制大丽黄萎病菌中微菌核的形成
DOI：
10.1094/mpmi-08-18-0233-r
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发表时间：
2018
影响因子：
3.5
通讯作者：
Wang Yonglin
中科院分区：
文献类型：
--
作者：
Yu Dimei;Fang Yulin;Tang Chen;Klosterman Steven;Tian Chengming;Wang Yonglin
大丽轮枝菌（Verticilliumdahliae）是一种土传真菌，是世界范围内引起植物枯萎病的主要病原菌。该真菌产生黑化微菌核，这对V的生存和传播至关重要。大丽花没有既有效又环保的杀真菌剂可用于抑制真菌。以前，枯草芽孢杆菌C232是从土壤中分离出来的，并被证明能抑制微菌核的形成。大丽花在这项研究中，液相色谱结合质谱分析揭示了抗真菌物质实际上是脂肽的混合物。暴露于V。DahliaeTo这些脂肽导致菌丝肿胀、细胞溶解和黑色素相关基因的下调。微菌核发育过程中脂肽抑制转录组的RNA测序分析显示，5，974个基因（2，131个上调和3，843个下调）与非抑制条件相比差异表达。此外，基因本体富集分析显示，参与应激反应、细胞代谢过程和翻译的基因显著富集。此外，脂肽抑制与次级代谢、蛋白质催化剂和高渗透压甘油反应信号通路相关的基因的表达。总之，这些发现为B.枯草杆菌脂肽抑制微菌核形成。这里获得的转录组学见解可能有助于开发生物制剂来对抗黄萎病。
Verticillium dahliaeis a soilborne fungus and the primary causal agent of vascular wilt diseases worldwide. The fungus produces melanized microsclerotia that are crucially important for the survival and spread ofV. dahliae. There are no fungicides available that are both effective and environmentally friendly to suppress the fungus. Previously,Bacillus subtilisC232 was isolated from soil and was demonstrated to suppress microsclerotia formation inV. dahliae. In this study, liquid chromatography coupled with mass spectrometry revealed that the antifungal substance is actually a mixture of lipopeptides. Exposure ofV. dahliaeto these lipopeptides resulted in hyphal swelling, cell lysis, and downregulation of melanin-related genes. RNA sequencing analyses of the lipopeptide-suppressed transcriptome during microsclerotial development revealed that 5,974 genes (2,131 upregulated and 3,843 downregulated) were differentially expressed versus nonsuppressive conditions. Furthermore, gene ontology enrichment analyses revealed that genes involved in response to stress, cellular metabolic processes, and translation were significantly enriched. Additionally, the lipopeptides inhibited expression of genes associated with secondary metabolism, protein catabolism, and the high-osmolarity glycerol response signaling pathway. Together, these findings provide evidence for the mechanism by whichB. subtilislipopeptides suppress microsclerotia formation. The transcriptomic insight garnered here may facilitate the development of biological agents to combat Verticillium wilt.