Evidence for a new feminizing Wolbachia strain in the isopod Armadillidium vulgare:: evolutionary implications
Evidence for a new feminizing Wolbachia strain in the isopod Armadillidium vulgare:: evolutionary implications
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DOI：
10.1038/sj.hdy.6800482
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发表时间：
2004-07-01
期刊：
影响因子：
3.8
通讯作者：
Bouchon, D
中科院分区：
文献类型：
--
作者：
Cordaux, R;Michel-Salzat, A;Bouchon, D
沃尔巴克氏杆菌是一种母系遗传的细胞内α变形杆菌，可感染多种节肢动物。在常见的避孕药臭虫Armadillidium uggare中，已知的沃尔巴克氏菌株负责遗传雄性的女性化。我们对来自西欧、东欧、北非和北美的20个种群的沃尔巴克氏菌多样性进行了调查。通过wsp基因的变异，鉴定出一个新的Wolbachia菌株(WVulM)，该菌株与该寄主物种中已知的(WVulC)有较远的亲缘关系。没有发现有多重感染的个人。接种实验表明，新的wVulM菌株也能诱导普通拟青霉雌性化。然而，wVulC菌株的传播率高于wVulM菌株，并且是普通按蚊种群中分布最广的沃尔巴克氏菌。对沃尔巴克氏菌感染者的线粒体16SrDNA基因进行了测序，揭示了4个宿主谱系的存在。细菌菌株与其各自宿主线粒体系统发育的比较未能显示出一致性，表明沃尔巴克氏菌株在普通拟青霉种群内水平传播。
Wolbachia are intracellular maternally inherited alpha-Proteobacteria infecting a wide range of arthropods. In the common pill bug Armadillidium vulgare, the known Wolbachia strain is responsible for feminization of genetic males. We have investigated Wolbachia diversity in 20 populations of A. vulgare from west and east Europe, north Africa and north America. A new Wolbachia strain (wVulM) was identified through the variability of the wsp gene, distantly related to that previously known (wVulC) in this host species. No individual with multiple infections was detected. Inoculation experiments indicated that the new wVulM bacterial strain also induces feminization in A. vulgare. However, the wVulC strain showed a higher transmission rate than the wVulM strain and was the most geographically widespread Wolbachia in A. vulgare populations. Mitochondrial 16SrDNA gene sequencing was conducted in Wolbachia-infected individuals, revealing the occurrence of four host lineages. The comparison of bacterial strains and their respective host mitochondrial phylogenies failed to show concordance, indicating horizontal transmission of the Wolbachia strains within populations of A. vulgare.