A practical guide to enzymology
A practical guide to enzymology
复制标题
酶学实用指南
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
C. Suelter
中科院分区：
文献类型：
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作者：
C. Suelter
在这本书中提供的是指导方针和实用的建议，任何人与酶的工作。它就以下方面提供咨询意见：溶剂、盐、底物和变性剂如何影响酶的功能和稳定性;开发从不同组织中纯化酶的方案;应用不同的技术分离蛋白质-硫酸铵、聚乙二醇和有机溶剂分离-离子交换、染料配体和生物配体亲和色谱;通过测定分子量、等电点pH、氨基酸组成和序列、配体结合和酶动力学来表征蛋白质。基本指南的程序和提示操作的技术提供了沿着许多参考文献，提供了额外的细节和理论。处理电泳，凝胶渗透，酶动力学和结合数据的方法进行了详细的处理。各种蛋白质定量方法进行了描述，并比较其灵敏度。
Provided in this book are guidelines and practical advice for anyone working with enzymes. It offers advice on: how solvents, salts, substrates, and denaturants affect enzyme function and stability; developing a scheme for purifying an enzyme from different kinds of tissue; applying different techniques for fractionating proteins - ammonium sulfate, polyethylene glycol, and organic solvent fractionation - ion exchange, dye ligand, and bio-ligand affinity chromatography; characterizing proteins by determining molecular weight, isoelectric pH, amino acid composition and sequence, ligand binding and enzyme kinetics. Basic guidelines to a procedure and hints for operation of a technique are offered along with many references to the literature which provides additional details and theory. Methods for treating electrophoretic, gel permeation, enzyme kinetic and binding data are treated in detail. Various protein quantitation methods are described and their sensitivities are compared.