Development of a thermosensitive hydrogel loaded with DTT and SDF-1 facilitating in situ periodontal bone regeneration
Development of a thermosensitive hydrogel loaded with DTT and SDF-1 facilitating in situ periodontal bone regeneration
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DOI：
10.1016/j.cej.2021.134308
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发表时间：
2021-12
影响因子：
15.1
通讯作者：
Shiyue Liu;Ya-nan Wang;Lu Yu;Jianhua Li;S. Ge
中科院分区：
文献类型：
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作者：
Shiyue Liu;Ya-nan Wang;Lu Yu;Jianhua Li;S. Ge
牙周炎是以病原微生物定植引起的局部炎症反应和牙槽骨吸收为特征的疾病。活性氧（ROS）水平的增加不仅加剧了局部炎症，而且抑制了骨生成过程。降低ROS水平和重塑成骨细胞活性是促进牙周组织原位再生的先决条件。在本研究中，我们开发了一种负载二硫苏糖醇（DTT）和基质细胞衍生因子-1（SDF-1）的温敏水凝胶，并探讨了其重塑成骨细胞活性和促进牙周骨再生的作用。在体外，水凝胶PEGD@SDF-1显著降低细胞ROS水平，重新激活成骨细胞的Wnt/β-catenin通路，恢复其在炎症状态下的成骨能力。在体内，将PEGD@SDF-1水凝胶应用于大鼠牙周炎模型，在降低ROS损伤水平、重新激活Wnt/β-catenin通路、减轻炎症水平的同时，还能促进牙周骨再生。综上所述，这些结果表明DTT和SDF-1的联合应用促进了牙周组织的原位再生，并且温敏水凝胶PEGD@SDF-1是牙周治疗的有希望的候选者。
Periodontitis is characterized by local inflammatory response and alveolar bone resorption induced by pathogenic microorganisms colonization. The increase in reactive oxygen species (ROS) levels not only aggravates the local inflammation, but also inhibits the process of osteogenesis. The coupled process of reducing ROS level and reshaping the activity of osteoblasts is prerequisite to facilitatein situperiodontal tissue regeneration. In this study, we developed a thermosensitive hydrogel loaded with dithiothreitol (DTT) and stromal cell-derived factor-1 (SDF-1) and explored its effect on reshaping osteoblasts activity and facilitating periodontal bone regeneration.In vitro, the hydrogel PEGD@SDF-1 significantly reduced cellular ROS levels, reactivated the Wnt/β-catenin pathway of osteoblasts and restored their osteogenic ability in an inflammatory state.In vivo, the hydrogel PEGD@SDF-1 was applied in rat periodontitis models and showed satisfactory effects on reducing the level of ROS damage, reactivating the Wnt/β-catenin pathway, alleviating the inflammation level, and promoting the periodontal bone regeneration, simultaneously. Taken together, these results suggest that the combined application of DTT and SDF-1 facilitatesin situperiodontal tissue regeneration and the thermosensitive hydrogel PEGD@SDF-1 is a promising candidate for periodontal therapy.