An S-ribonuclease binding protein EBS1 and brassinolide signaling are specifically required for Arabidopsis tolerance to bicarbonate
An S-ribonuclease binding protein EBS1 and brassinolide signaling are specifically required for Arabidopsis tolerance to bicarbonate
复制标题
拟南芥对碳酸氢盐的耐受性特别需要 S-核糖核酸酶结合蛋白 EBS1 和油菜素内酯信号传导
DOI：
10.1093/jxb/eraa524
复制
发表时间：
2021-02-24
影响因子：
6.9
通讯作者：
Liu, Shenkui
中科院分区：
文献类型：
--
作者：
Chen, Jipeng;Li, Xiaoxiao;Liu, Shenkui
土壤中存在的碳酸氢盐（NaHCO₃）通常被认为是对植物的一种混合胁迫，伴有盐分和高pH值。植物中针对NaHCO₃的特异性信号传导鲜有报道。在本研究中，为了确定拟南芥中针对NaHCO₃的特异性信号传导，进行了转录组分析。通过与乙酸盐处理对比，进行加权相关网络分析以分离出针对NaHCO₃的特异性模块。对在NaHCO₃ - 根特异性模块中与乙酸钠处理表达相反的基因，利用其相应的基因敲除突变体进行了进一步检测。编码一种S - 核糖核酸酶结合蛋白的基因“碳酸氢盐特异敏感1（EBS1）”被确定为特异性参与植物对NaHCO₃的耐受性，而不参与对另外两种碱性盐（乙酸盐和磷酸盐）的耐受性。我们还确定了受碳酸氢盐、乙酸盐和磷酸盐共同调控的基因。在这些基因中，多个与油菜素内酯相关的基因本体术语得到富集。遗传学分析表明，油菜素内酯信号传导正向调控植物对NaHCO₃胁迫的耐受性，但负向调控对乙酸盐和磷酸盐的耐受性。总体而言，我们的数据确定了碳酸氢盐特异性基因，并证实碱性胁迫主要取决于弱酸离子的特异性，而非高pH值。
Bicarbonate (NaHCO3) present in soils is usually considered to be a mixed stress for plants, with salts and high pH. NaHCO3-specific signaling in plants has rarely been reported. In this study, transcriptome analyses were conducted in order to identify NaHCO3-specific signaling in Arabidopsis. Weighted correlation network analysis was performed to isolate NaHCO3-specific modules in comparison with acetate treatment. The genes in the NaHCO3-root-specific module, which exhibited opposite expression to that in sodium acetate treatments, were further examined with their corresponding knock-out mutants. The gene Exclusively Bicarbonate Sensitive 1 (EBS1) encoding an S-ribonuclease binding protein, was identified to be specifically involved in plant tolerance to NaHCO3, but not to the other two alkaline salts, acetate and phosphate. We also identified the genes that are commonly regulated by bicarbonate, acetate and phosphate. Multiple brassinosteroid-associated gene ontology terms were enriched in these genes. Genetic assays showed that brassinosteroid signaling positively regulated plant tolerance to NaHCO3 stress, but negatively regulated tolerance to acetate and phosphate. Overall, our data identified bicarbonate-specific genes, and confirmed that alkaline stress is mainly dependent on the specificities of the weak acid ions, rather than high pH.