In Situ Visualization of Block Copolymer Self-Assembly in Organic Media by Super-Resolution Fluorescence Microscopy.
In Situ Visualization of Block Copolymer Self-Assembly in Organic Media by Super-Resolution Fluorescence Microscopy.
复制标题
DOI：
10.1002/chem.201504100
复制
发表时间：
2015-12-14
期刊：
影响因子：
--
通讯作者：
Manners I
中科院分区：
文献类型：
--
作者：
Boott CE;Laine RF;Mahou P;Finnegan JR;Leitao EM;Webb SE;Kaminski CF;Manners I
非常需要能够可视化源自有机溶剂中的溶液自组装过程的纳米材料的分析方法。在本文中，我们展示了使用受激发射耗尽显微镜（STED）和单分子定位显微镜（SMLM）在亚衍射尺度下映射有机介质中的活性结晶驱动嵌段共聚物（BCP）自组装。四种不同的染料被成功地用于BCP纳米结构的单色超分辨率成像，允许原位确定胶束长度分布。使用双色SMLM成像来测量和比较红色荧光BCP添加到绿色荧光种子胶束末端以产生嵌段共胶束的速率。虽然水溶液体系已经建立，但结果突出了超分辨率显微镜技术在有机介质中自组装过程研究中的潜力。
Analytical methods that enable visualization of nanomaterials derived from solution self‐assembly processes in organic solvents are highly desirable. Herein, we demonstrate the use of stimulated emission depletion microscopy (STED) and single molecule localization microscopy (SMLM) to map living crystallization‐driven block copolymer (BCP) self‐assembly in organic media at the sub‐diffraction scale. Four different dyes were successfully used for single‐colour super‐resolution imaging of the BCP nanostructures allowing micelle length distributions to be determined in situ. Dual‐colour SMLM imaging was used to measure and compare the rate of addition of red fluorescent BCP to the termini of green fluorescent seed micelles to generate block comicelles. Although well‐established for aqueous systems, the results highlight the potential of super‐resolution microscopy techniques for the interrogation of self‐assembly processes in organic media.