Bactericidal effect of hydroxyl radicals generated by the sonolysis and photolysis of hydrogen peroxide for endodontic applications
Bactericidal effect of hydroxyl radicals generated by the sonolysis and photolysis of hydrogen peroxide for endodontic applications
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DOI：
10.1016/j.micpath.2016.12.010
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发表时间：
2017-02-01
影响因子：
3.8
通讯作者：
Ogiso, Bunnai
中科院分区：
文献类型：
--
作者：
Ibi, Haruna;Hayashi, Makoto;Ogiso, Bunnai
根管治疗的目的是从感染的牙根管中清除细菌及其产物。为了有效地消毒根管，超声冲洗系统，其中羟基自由基（HO '）产生的H2 O2的超声分解人工，先前开发的牙髓应用，并证明有杀菌效果对粪肠球菌。为了改进该系统，我们研究了由H2 O2产生的HO'的体外杀菌效果，通过同时照射超声进行声分解和牙科LED光进行光解，峰值波长为405 nm。关于LED照射，使用了两种方法：（i）“理想”实验条件（靠近玻璃管照射），和（ii）模拟牙髓条件（掩蔽玻璃管的更远照射）。在此条件下，通过电子自旋共振（ESR）光谱检测H2 O2产生HO'，并测定对大肠杆菌的杀菌效果。通过测量殖民地形成单位（CFU）/mL来评估粪球菌。结果表明，用ESR法测定HO的生成量，并对大肠杆菌有杀菌作用。在两种条件下，通过使用CFU/mL的活菌计数的粪肠球菌以时间依赖性方式显著增强。在这些条件的比较中，在“理想”实验条件下的杀菌活性与在模拟牙髓条件下的杀菌活性相似。此外，有效杀灭E.在模拟牙髓条件下，通过超声降解和H2 O2光解的粪便比单独超声降解短。结果表明，超声和LED灯激活的H2 O2可能是一种安全有效的根管消毒技术。(C)2016爱思唯尔有限公司版权所有
The aim of endodontic root canal treatment is the elimination of bacteria and their products from an infected tooth root canal. To effectively disinfect a root canal, an ultrasonic irrigation system, in which hydroxyl radicals (HO') generated artificially by sonolysis of H2O2, was developed previously for endodontic applications and was demonstrated to have bactericidal efficacy against Enterococcus faecalis. To improve this system, we examined the in vitro bactericidal effects of HO' generated from H2O2, activated by simultaneous irradiation with ultrasound for sonolysis and dental LED light for photolysis with a peak wavelength of 405 nm. Regarding the LED irradiation, two methods were used: (i) 'ideal' experimental conditions (irradiation close to the glass tube), and (ii) simulated endodontic conditions (more distant irradiation of a masked glass tube). In these conditions, HO' generation from H2O2 was detected by electron spin resonance (ESR) spectroscopy, and bactericidal efficacy against E. faecalis was assessed by measuring the colony forming units (CFU)/mL. The results indicated that HO generation by ESR measurements and the bactericidal effect on E. faecalis by viable count using CFU/mL were enhanced significantly in a time-dependent manner in both conditions. In a comparison of these conditions, bactericidal activity under 'ideal' experimental conditions was similar to that under simulated endodontic conditions. Moreover, the irradiation time for effective killing of E. faecalis through the sonolysis and photolysis of H2O2 under simulated endodontic conditions was shorter than that with sonolysis alone. These results demonstrate that H2O2 activated by ultrasound and LED light may be a safe and effective disinfection technique for endodontic root canal treatment. (C) 2016 Elsevier Ltd. All rights reserved.